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LPCAT3 Rabbit pAb (A17604)

Publications (10) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - LPCAT3 Rabbit pAb (A17604)

Western blot analysis of lysates from HepG2 cells, using LPCAT3 Rabbit pAb (A17604) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - LPCAT3 Rabbit pAb (A17604)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using LPCAT3 Rabbit pAb (A17604) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - LPCAT3 Rabbit pAb (A17604)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using LPCAT3 Rabbit pAb (A17604) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - LPCAT3 Rabbit pAb (A17604)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using LPCAT3 Rabbit pAb (A17604) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product nameLPCAT3 Rabbit pAb
Catalog No.A17604
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables 1-acylglycerophosphocholine O-acyltransferase activity; 1-acylglycerophosphoethanolamine O-acyltransferase activity; and 1-acylglycerophosphoserine O-acyltransferase activity. Involved in phosphatidylcholine acyl-chain remodeling; phosphatidylethanolamine acyl-chain remodeling; and phosphatidylserine acyl-chain remodeling. Located in endoplasmic reticulum membrane.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 200-300 of human LPCAT3 (NP_005759.4).
SequenceGPQFSMNHYMKLVQGELIDIPGKIPNSIIPALKRLSLGLFYLVGYTLLSPHITEDYLLTEDYDNHPFWFRCMYMLIWGKFVLYKYVTCWLVTEGVCILTGL
Gene ID
Swiss Prot
SynonymsC3F; LPCAT; LPSAT; OACT5; nessy; MBOAT5; LPLAT 5; LPLAT12; LPCAT3
Calculated MW56kDa
Observed MW56kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHepG2
Cellular location
Customer validation

WB(Rattus norvegicus, Mus musculus, Homo sapiens, Other)

Other(Mus musculus)

Documents

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ABclonal:Western blot - LPCAT3 Rabbit pAb (A17604)}

Western blot - LPCAT3 Rabbit pAb (A17604)

Western blot analysis of lysates from HepG2 cells, using LPCAT3 Rabbit pAb (A17604) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - LPCAT3 Rabbit pAb (A17604)}

Immunohistochemistry - LPCAT3 Rabbit pAb (A17604)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using LPCAT3 Rabbit pAb (A17604) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - LPCAT3 Rabbit pAb (A17604)}

Immunohistochemistry - LPCAT3 Rabbit pAb (A17604)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using LPCAT3 Rabbit pAb (A17604) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - LPCAT3 Rabbit pAb (A17604)}

Immunohistochemistry - LPCAT3 Rabbit pAb (A17604)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using LPCAT3 Rabbit pAb (A17604) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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