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LRPPRC Rabbit pAb (A3365)

Publications (7) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - LRPPRC Rabbit pAb (A3365)

Western blot analysis of various lysates, using LRPPRC Rabbit pAb (A3365) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.

ABclonal:Western blot - LRPPRC Rabbit pAb (A3365)

Western blot analysis of various lysates, using LRPPRC Rabbit pAb (A3365) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - LRPPRC Rabbit pAb (A3365)

Immunohistochemistry analysis of paraffin-embedded human breast cancer using LRPPRC Rabbit pAb (A3365) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - LRPPRC Rabbit pAb (A3365)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using LRPPRC Rabbit pAb (A3365) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - LRPPRC Rabbit pAb (A3365)

Confocal immunofluorescence analysis of Hela cells using LRPPRC Rabbit pAb (A3365) at dilution of 1:400. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - LRPPRC Rabbit pAb (A3365)

Immunofluorescence analysis of A-549 cells using LRPPRC Rabbit pAb (A3365) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - LRPPRC Rabbit pAb (A3365)

Immunofluorescence analysis of HepG2 cells using LRPPRC Rabbit pAb (A3365) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - LRPPRC Rabbit pAb (A3365)

Immunofluorescence analysis of NIH/3T3 cells using LRPPRC Rabbit pAb (A3365) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameLRPPRC Rabbit pAb
Catalog No.A3365
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a leucine-rich protein that has multiple pentatricopeptide repeats (PPR). The precise role of this protein is unknown but studies suggest it may play a role in cytoskeletal organization, vesicular transport, or in transcriptional regulation of both nuclear and mitochondrial genes. The protein localizes primarily to mitochondria and is predicted to have an N-terminal mitochondrial targeting sequence. Mutations in this gene are associated with the French-Canadian type of Leigh syndrome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1041-1394 of human LRPPRC (NP_573566.2).
SequenceIACRLNQKKGAYDIFLNAKEQNIVFNAETYSNLIKLLMSEDYFTQAMEVKAFAETHIKGFTLNDAANSRLIITQVRRDYLKEAVTTLKTVLDQQQTPSRLAVTRVIQALAMKGDVENIEVVQKMLNGLEDSIGLSKMVFINNIALAQIKNNNIDAAIENIENMLTSENKVIEPQYFGLAYLFRKVIEEQLEPAVEKISIMAERLANQFAIYKPVTDFFLQLVDAGKVDDARALLQRCGAIAEQTPILLLFLLRNSRKQGKASTVKSVLELIPELNEKEEAYNSLMKSYVSEKDVTSAKALYEHLTAKNTKLDDLFLKRYASLLKYAGEPVPFIEPPESFEFYAQQLRKLRENSS
Gene ID
Swiss Prot
SynonymsLSFC; GP130; LRP130; MC4DN5; CLONE-23970; LRPPRC
Calculated MW158kDa
Observed MW158kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:100 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHepG2, HeLa, Mouse heart, Rat heart, Rat brain
Cellular locationMitochondrion, Nucleus, Nucleus inner membrane, Nucleus outer membrane, nucleoplasm
Customer validation

WB(Mus musculus, Homo sapiens)

IHC(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - LRPPRC Rabbit pAb (A3365)}

Western blot - LRPPRC Rabbit pAb (A3365)

Western blot analysis of various lysates, using LRPPRC Rabbit pAb (A3365) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.
ABclonal:Western blot - LRPPRC Rabbit pAb (A3365)}

Western blot - LRPPRC Rabbit pAb (A3365)

Western blot analysis of various lysates, using LRPPRC Rabbit pAb (A3365) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - LRPPRC Rabbit pAb (A3365)}

Immunohistochemistry - LRPPRC Rabbit pAb (A3365)

Immunohistochemistry analysis of paraffin-embedded human breast cancer using LRPPRC Rabbit pAb (A3365) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - LRPPRC Rabbit pAb (A3365)}

Immunohistochemistry - LRPPRC Rabbit pAb (A3365)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using LRPPRC Rabbit pAb (A3365) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - LRPPRC Rabbit pAb (A3365)}

Immunofluorescence - LRPPRC Rabbit pAb (A3365)

Confocal immunofluorescence analysis of Hela cells using LRPPRC Rabbit pAb (A3365) at dilution of 1:400. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - LRPPRC Rabbit pAb (A3365)}

Immunofluorescence - LRPPRC Rabbit pAb (A3365)

Immunofluorescence analysis of A-549 cells using LRPPRC Rabbit pAb (A3365) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - LRPPRC Rabbit pAb (A3365)}

Immunofluorescence - LRPPRC Rabbit pAb (A3365)

Immunofluorescence analysis of HepG2 cells using LRPPRC Rabbit pAb (A3365) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - LRPPRC Rabbit pAb (A3365)}

Immunofluorescence - LRPPRC Rabbit pAb (A3365)

Immunofluorescence analysis of NIH/3T3 cells using LRPPRC Rabbit pAb (A3365) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)