제품 > 항체 > 다중클론항체(pAb)

LRRTM4 Rabbit pAb (A15898)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - LRRTM4 Rabbit pAb (A15898)

Western blot analysis of various lysates using LRRTM4 Rabbit pAb (A15898) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameLRRTM4 Rabbit pAb
Catalog No.A15898
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable heparan sulfate proteoglycan binding activity. Predicted to be involved in regulation of synapse assembly. Predicted to act upstream of or within AMPA glutamate receptor clustering; positive regulation of synapse assembly; and regulation of presynaptic membrane organization. Predicted to be located in postsynaptic membrane. Predicted to be part of AMPA glutamate receptor complex. Predicted to be active in extracellular matrix; extracellular space; and glutamatergic synapse. Predicted to be integral component of postsynaptic density membrane.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 310-420 of human LRRTM4 (NP_001269857.1).
SequenceSGNMWECSRSICPLFYWLKNFKGNKESTMICAGPKHIQGEKVSDAVETYNICSEVQVVNTERSHLVPQTPQKPLIIPRPTIFKPDVTQSTFETPSPSPGFQIPGAEQEYEH
Gene ID
Swiss Prot
SynonymsLRRTM4
Calculated MW67kDa
Observed MW80kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesU-87MG, Mouse brain, Rat brain
Cellular locationCell junction, Cell membrane, Single-pass type I membrane protein, postsynaptic cell membrane, synapse
Customer validation

WB(Homo sapiens)

    ABclonal:Western blot - LRRTM4 Rabbit pAb (A15898)}

    Western blot - LRRTM4 Rabbit pAb (A15898)

    Western blot analysis of various lysates using LRRTM4 Rabbit pAb (A15898) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A15898? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)