Western blot - MCCC2 Rabbit mAb (A26776)
Western blot analysis of various lysates using MCCC2 Rabbit mAb (A26776) at 1:20000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): MOLT-4
Exposure time: 20s.
Western blot - MCCC2 Rabbit mAb (A26776)
Western blot analysis of various lysates using MCCC2 Rabbit mAb (A26776) at 1:20000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Immunohistochemistry - MCCC2 Rabbit mAb (A26776)
Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using MCCC2 Rabbit mAb (A26776) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry - MCCC2 Rabbit mAb (A26776)
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using MCCC2 Rabbit mAb (A26776) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry - MCCC2 Rabbit mAb (A26776)
Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using MCCC2 Rabbit mAb (A26776) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry - MCCC2 Rabbit mAb (A26776)
Immunohistochemistry analysis of paraffin-embedded Human liver tissue using MCCC2 Rabbit mAb (A26776) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry - MCCC2 Rabbit mAb (A26776)
Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue using MCCC2 Rabbit mAb (A26776) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunofluorescence - MCCC2 Rabbit mAb (A26776)
Confocal imaging of HeLa cells using MCCC2 Rabbit mAb (A26776, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Immunofluorescence - MCCC2 Rabbit mAb (A26776)
Confocal imaging of MCF7 cells using MCCC2 Rabbit mAb (A26776, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.