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MEF2D Rabbit pAb (A16398)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - MEF2D Rabbit pAb (A16398)

Western blot analysis of various lysates using MEF2D Rabbit pAb (A16398) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - MEF2D Rabbit pAb (A16398)

Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using MEF2D Rabbit pAb (A16398) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - MEF2D Rabbit pAb (A16398)

Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using MEF2D Rabbit pAb (A16398) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product nameMEF2D Rabbit pAb
Catalog No.A16398
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the myocyte-specific enhancer factor 2 (MEF2) family of transcription factors. Members of this family are involved in control of muscle and neuronal cell differentiation and development, and are regulated by class II histone deacetylases. Fusions of the encoded protein with Deleted in Azoospermia-Associated Protein 1 (DAZAP1) due to a translocation have been found in an acute lymphoblastic leukemia cell line, suggesting a role in leukemogenesis. The encoded protein may also be involved in Parkinson disease and myotonic dystrophy. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 431-521 of human MEF2D (NP_005911.1).
SequenceTTHPHISIKSEPVSPSRERSPAPPPPAVFPAARPEPGDGLSSPAGGSYETGDRDDGRGDFGPTLGLLRPAPEPEAEGSAVKRMRLDTWTLK
Gene ID
Swiss Prot
SynonymsMEF2D
Calculated MW56kDa
Observed MW54kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, U-251MG, Mouse Brain
Cellular locationNucleus.

Documents

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ABclonal:Western blot - MEF2D Rabbit pAb (A16398)}

Western blot - MEF2D Rabbit pAb (A16398)

Western blot analysis of various lysates using MEF2D Rabbit pAb (A16398) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - MEF2D Rabbit pAb (A16398)}

Immunohistochemistry - MEF2D Rabbit pAb (A16398)

Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using MEF2D Rabbit pAb (A16398) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - MEF2D Rabbit pAb (A16398)}

Immunohistochemistry - MEF2D Rabbit pAb (A16398)

Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using MEF2D Rabbit pAb (A16398) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)