제품 > 항체 > 단일클론항체(mAb)

MSH2 Rabbit mAb (A8740)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - MSH2 Rabbit mAb (A8740)

Western blot analysis of various lysates using MSH2 Rabbit mAb (A8740) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - MSH2 Rabbit mAb (A8740)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using MSH2 Rabbit mAb (A8740) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - MSH2 Rabbit mAb (A8740)

Immunohistochemistry analysis of paraffin-embedded Human colon using MSH2 Rabbit mAb (A8740) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Overview

Product nameMSH2 Rabbit mAb
Catalog No.A8740
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1285
This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human MSH2 (P43246).
SequenceMAVQPKETLQLESAAEVGFVRFFQGMPEKPTTTVRLFDRGDFYTAHGEDALLAAREVFKTQGVIKYMGPAGAKNLQSVVLSKMNFESFVKDLLLVRQYRV
Gene ID
Swiss Prot
SynonymsFCC1; COCA1; HNPCC; LCFS2; MSH-2; hMSH2; HNPCC1; LYNCH1; MMRCS2; MSH2
Calculated MW105kDa
Observed MW105kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, 293T, A549, NIH/3T3, C6, Mouse testis
Cellular locationNucleus
Customer validation

WB(Other)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - MSH2 Rabbit mAb (A8740)}

Western blot - MSH2 Rabbit mAb (A8740)

Western blot analysis of various lysates using MSH2 Rabbit mAb (A8740) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - MSH2 Rabbit mAb (A8740)}

Immunohistochemistry - MSH2 Rabbit mAb (A8740)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using MSH2 Rabbit mAb (A8740) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - MSH2 Rabbit mAb (A8740)}

Immunohistochemistry - MSH2 Rabbit mAb (A8740)

Immunohistochemistry analysis of paraffin-embedded Human colon using MSH2 Rabbit mAb (A8740) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A8740? Please let us know so that we can cite the reference in this datasheet.

항체 (3)

Secondary Antibodies (26)