제품 > 항체 > 다중클론항체(pAb)

MSH3 Rabbit pAb (A5681)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - MSH3 Rabbit pAb (A5681)

Western blot analysis of extracts of SW620 cells, using MSH3 Rabbit pAb (A5681) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product nameMSH3 Rabbit pAb
Catalog No.A5681
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene forms a heterodimer with MSH2 to form MutS beta, part of the post-replicative DNA mismatch repair system. MutS beta initiates mismatch repair by binding to a mismatch and then forming a complex with MutL alpha heterodimer. This gene contains a polymorphic 9 bp tandem repeat sequence in the first exon. The repeat is present 6 times in the reference genome sequence and 3-7 repeats have been reported. Defects in this gene are a cause of susceptibility to endometrial cancer.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 617-761 of human MSH3 (NP_004696.2).
SequenceFNTSEEHHADMYRSDLPNPDTLSAELHCWRIKWKHRGKDIELPSTIYEALHLPDIKFFPNVYALLKVLCILPVMKVENERYENGRKRLKAYLRNTLTDQRSSNLALLNINFDIKHDLDLMVDTYIKLYTSKSELPTDNSETVENT
Gene ID
Swiss Prot
SynonymsDUP; FAP4; MRP1
Calculated MW127kDa
Observed MW127kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesSW620
Cellular locationnucleoplasm, nucleus
Customer validation

IHC(Ctenopharyngodon idellus)

WB(Ctenopharyngodon idellus)

Documents

Certificate of Compliance

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ABclonal:Western blot - MSH3 Rabbit pAb (A5681)}

Western blot - MSH3 Rabbit pAb (A5681)

Western blot analysis of extracts of SW620 cells, using MSH3 Rabbit pAb (A5681) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)