제품 > 항체 > 다중클론항체(pAb)

MST1/STK4 Rabbit pAb (A8043)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using MST1/STK4 Rabbit pAb (A8043) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

ABclonal:Immunoprecipitation - MST1/STK4 Rabbit pAb (A8043)

Immunoprecipitation analysis of 100 μg extracts of HepG2 cells using 3 μg MST1/STK4 antibody (A8043). Western blot was performed from the immunoprecipitate using MST1/STK4 antibody (A8043) at a dilution of 1:1000.

Overview

Product name	MST1/STK4 Rabbit pAb
Catalog No.	A8043
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 303-487 of human MST1/STK4 (NP_006273.1).
Sequence	KRQESQQREVDQDDEENSEEDEMDSGTMVRAVGDEMGTVRVASTMTDGANTMIEHDDTLPSQLGTMVINAEDEEEEGTMKRRDETMQPAKPSFLEYFEQKEKENQINSFGKSVPGPLKNSSDWKIPQDGDYEFLKSWTVEDLQKRLLALDPMMEQEIEEIRQKYQSKRQPILDAIEAKKRRQQNF
Gene ID
Swiss Prot
Synonyms	KRS2; MST1; YSK3; K4
Calculated MW	56kDa
Observed MW	56kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	U-87MG, HepG2, HeLa, MCF-7, Mouse brain, Mouse lung, Mouse spleen, Rat liver
Cellular location	Cytoplasm, Nucleus
Customer validation	IF(Homo sapiens) WB(Mus musculus, Homo sapiens, Drosophila melanogaster) IHC(Homo sapiens) Co-IP(Drosophila melanogaster)

Documents

Certificate of Compliance

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Lot number

Research Area

MST1/STK4 Rabbit pAb images

Western blot - MST1/STK4 Rabbit pAb (A8043)

Immunoprecipitation - MST1/STK4 Rabbit pAb (A8043)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for MST1/STK4 Rabbit pAb (A8043)

Product:

MST1/STK4 Rabbit pAb

Journal:

Human reproduction

Application:

IF:

6.918

Species:

Homo sapiens

PMID:

26848187

Title:

Protein kinase A inhibitor, H89, enhances survival and clonogenicity of dissociated human embryonic stem cells through Rho-associated coiled-coil containing protein kinase (ROCK) inhibition

References for MST1/STK4 Rabbit pAb (A8043)

Product:

MST1/STK4 Rabbit pAb

Journal:

Journal of bioenergetics and biomembranes

Application:

IF:

3.85

Species:

Mus musculus

PMID:

36562913