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MTCO2 Rabbit mAb (A3843)

Publication (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - MTCO2 Rabbit mAb (A3843)

Western blot analysis of various lysates using MTCO2 Rabbit mAb (A3843) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - MTCO2 Rabbit mAb (A3843)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using MTCO2 Rabbit mAb (A3843) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - MTCO2 Rabbit mAb (A3843)

Immunohistochemistry analysis of paraffin-embedded Human brain tissue using MTCO2 Rabbit mAb (A3843) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - MTCO2 Rabbit mAb (A3843)

Confocal imaging of paraffin-embedded Human liver tissue using MTCO2 Rabbit mAb (A3843, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - MTCO2 Rabbit mAb (A3843)

Confocal imaging of HeLa cells using MTCO2 Rabbit mAb (A3843, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product nameMTCO2 Rabbit mAb
Catalog No.A3843
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0844
Contributes to cytochrome-c oxidase activity. Predicted to be involved in mitochondrial electron transport, cytochrome c to oxygen and positive regulation of vasoconstriction. Located in mitochondrial inner membrane. Part of respiratory chain complex IV. Biomarker of Huntington's disease and stomach cancer.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 128-227 of human MTCO2 (P00403).
SequenceLEPGDLRLLDVDNRVVLPIEAPIRMMITSQDVLHSWAVPTLGLKTDAIPGRLNQTTFTATRPGVYYGQCSEICGANHSFMPIVLELIPLKIFEMGPVFTL
Gene ID
Swiss Prot
SynonymsCOII; MTCO2; COX2
Calculated MW26kDa
Observed MW19kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • IHC-P 1:200 - 1:800
  • IF/ICC 1:200 - 1:800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, HepG2, K-562
Cellular locationMitochondrion inner membrane, Multi-pass membrane protein.
Customer validation

WB(Mus musculus, Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - MTCO2 Rabbit mAb (A3843)}

Western blot - MTCO2 Rabbit mAb (A3843)

Western blot analysis of various lysates using MTCO2 Rabbit mAb (A3843) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - MTCO2 Rabbit mAb (A3843)}

Immunohistochemistry - MTCO2 Rabbit mAb (A3843)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using MTCO2 Rabbit mAb (A3843) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - MTCO2 Rabbit mAb (A3843)}

Immunohistochemistry - MTCO2 Rabbit mAb (A3843)

Immunohistochemistry analysis of paraffin-embedded Human brain tissue using MTCO2 Rabbit mAb (A3843) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - MTCO2 Rabbit mAb (A3843)}

Immunofluorescence - MTCO2 Rabbit mAb (A3843)

Confocal imaging of paraffin-embedded Human liver tissue using MTCO2 Rabbit mAb (A3843, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - MTCO2 Rabbit mAb (A3843)}

Immunofluorescence - MTCO2 Rabbit mAb (A3843)

Confocal imaging of HeLa cells using MTCO2 Rabbit mAb (A3843, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A3843? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)