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MTCO2 Rabbit mAb (A3843)

Publication (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - MTCO2 Rabbit mAb (A3843)

Western blot analysis of various lysates using MTCO2 Rabbit mAb (A3843) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - MTCO2 Rabbit mAb (A3843)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using MTCO2 Rabbit mAb (A3843) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - MTCO2 Rabbit mAb (A3843)

Immunohistochemistry analysis of paraffin-embedded Human brain tissue using MTCO2 Rabbit mAb (A3843) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - MTCO2 Rabbit mAb (A3843)

Confocal imaging of paraffin-embedded Human liver tissue using MTCO2 Rabbit mAb (A3843, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - MTCO2 Rabbit mAb (A3843)

Confocal imaging of HeLa cells using MTCO2 Rabbit mAb (A3843, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product nameMTCO2 Rabbit mAb
Catalog No.A3843
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0844
Contributes to cytochrome-c oxidase activity. Predicted to be involved in mitochondrial electron transport, cytochrome c to oxygen and positive regulation of vasoconstriction. Located in mitochondrial inner membrane. Part of respiratory chain complex IV. Biomarker of Huntington's disease and stomach cancer.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 128-227 of human MTCO2 (P00403).
SequenceLEPGDLRLLDVDNRVVLPIEAPIRMMITSQDVLHSWAVPTLGLKTDAIPGRLNQTTFTATRPGVYYGQCSEICGANHSFMPIVLELIPLKIFEMGPVFTL
Gene ID
Swiss Prot
SynonymsCOII; MTCO2; COX2
Calculated MW26kDa
Observed MW19kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • IHC-P 1:200 - 1:800
  • IF/ICC 1:200 - 1:800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, HepG2, K-562
Cellular locationMitochondrion inner membrane, Multi-pass membrane protein.
Customer validation

WB(Mus musculus, Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - MTCO2 Rabbit mAb (A3843)}

Western blot - MTCO2 Rabbit mAb (A3843)

Western blot analysis of various lysates using MTCO2 Rabbit mAb (A3843) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - MTCO2 Rabbit mAb (A3843)}

Immunohistochemistry - MTCO2 Rabbit mAb (A3843)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using MTCO2 Rabbit mAb (A3843) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - MTCO2 Rabbit mAb (A3843)}

Immunohistochemistry - MTCO2 Rabbit mAb (A3843)

Immunohistochemistry analysis of paraffin-embedded Human brain tissue using MTCO2 Rabbit mAb (A3843) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - MTCO2 Rabbit mAb (A3843)}

Immunofluorescence - MTCO2 Rabbit mAb (A3843)

Confocal imaging of paraffin-embedded Human liver tissue using MTCO2 Rabbit mAb (A3843, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - MTCO2 Rabbit mAb (A3843)}

Immunofluorescence - MTCO2 Rabbit mAb (A3843)

Confocal imaging of HeLa cells using MTCO2 Rabbit mAb (A3843, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A3843? Please let us know so that we can cite the reference in this datasheet.