제품 > 항체 > 다중클론항체(pAb)

MT-CO3 Rabbit pAb (A9939)

Publication (4) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - MT-CO3 Rabbit pAb (A9939)

Western blot analysis of extracts of various cell lines, using MT-CO3 antibody (A9939) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameMT-CO3 Rabbit pAb
Catalog No.A9939
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable electron transfer activity and oxidoreduction-driven active transmembrane transporter activity. Predicted to be involved in mitochondrial electron transport, cytochrome c to oxygen and respiratory chain complex IV assembly. Located in mitochondrion. Is expressed in several structures, including alimentary system; brain; heart; integumental system; and sensory organ. Human ortholog(s) of this gene implicated in MELAS syndrome. Orthologous to human MT-CO3 (mitochondrially encoded cytochrome c oxidase III).
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of mouse MT-CO3 (NP_904334.1).
SequenceMTHQTHAYHMVNPSPWPLTGAFSALLLTSGLVMWFHYNSITLLTLGLLTNILTMYQWWRDVIREGTYQGHHTPIVQKGLRYGMILFIVSEVFFFAGFFWA
Gene ID
Swiss Prot
SynonymsCOX3
Calculated MW29kDa
Observed MW30kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse liver, Rat liver
Cellular locationMitochondrion inner membrane, Multi-pass membrane protein
Customer validation

WB(Rattus norvegicus, Homo sapiens, Gallus gallus)

Documents

Certificate of Compliance

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    ABclonal:Western blot - MT-CO3 Rabbit pAb (A9939)}

    Western blot - MT-CO3 Rabbit pAb (A9939)

    Western blot analysis of extracts of various cell lines, using MT-CO3 antibody (A9939) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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