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MT-ND3 Rabbit pAb (A17969)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using MT-ND3 Rabbit pAb (A17969) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - MT-ND3 Rabbit pAb (A17969)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using MT-ND3 Rabbit pAb (A17969) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human appendix using MT-ND3 Rabbit pAb (A17969) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using MT-ND3 Rabbit pAb (A17969) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - MT-ND3 Rabbit pAb (A17969)

Immunofluorescence analysis of L929 cells using MT-ND3 Rabbit pAb (A17969) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HepG2 cells using MT-ND3 Rabbit pAb (A17969) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - MT-ND3 Rabbit pAb (A17969)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg MT-ND3 antibody (A17969). Western blot was performed from the immunoprecipitate using MT-ND3 antibody (A17969) at a dilution of 1:500.

Overview

Product name	MT-ND3 Rabbit pAb
Catalog No.	A17969
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Enables NADH dehydrogenase (ubiquinone) activity. Involved in mitochondrial electron transport, NADH to ubiquinone. Part of mitochondrial respiratory chain complex I. Implicated in Leber hereditary optic neuropathy; Leigh disease; and Parkinson's disease.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-70 of human MT-ND3 (YP_003024033.1).
Sequence	MNFALILMINTLLALLLMIITFWLPQLNGYMEKSTPYECGFDPMSPARVPFSMKFFLVAITFLLFDLEIA
Gene ID
Swiss Prot
Synonyms	MTND3; ND3; MT-ND3
Calculated MW	13kDa
Observed MW	15kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:100 - 1:500 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	293T, PC-3, Mouse heart, Mouse brain
Cellular location	mitochondrial inner membrane, mitochondrial respiratory chain complex I.
Customer validation	WB(Homo sapiens, Mus musculus, Mus musculus) Co-IP(Homo sapiens) WB(Homo sapiens)