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MT-ND4 Rabbit pAb (A9941)

Publications (13) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - MT-ND4 Rabbit pAb (A9941)

Western blot analysis of various lysates using MT-ND4 Rabbit pAb (A9941) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameMT-ND4 Rabbit pAb
Catalog No.A9941
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable NADH dehydrogenase (ubiquinone) activity and ubiquinone binding activity. Predicted to contribute to NADH dehydrogenase activity. Predicted to be involved in several processes, including electron transport coupled proton transport; mitochondrial electron transport, NADH to ubiquinone; and mitochondrial respiratory chain complex I assembly. Located in mitochondrion. Human ortholog(s) of this gene implicated in Leber hereditary optic neuropathy; Parkinson's disease; macular degeneration; and schizophrenia. Orthologous to human MT-ND4 (mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 4).
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 360-459 of mouse MT-ND4 (NP_904337.1).
SequenceLMASLANLALPPSINLMGELFITMSLFSWSNFTIILMGINIIITGMYSMYMIITTQRGKLTNHMINLQPSHTRELTLMALHMIPLILLTTSPKLITGLTM
Gene ID
Swiss Prot
SynonymsND4; MT-ND4
Calculated MW51kDa
Observed MW50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T, RD, Mouse heart, Rat heart
Cellular locationMitochondrion membrane, Multi-pass membrane protein.
Customer validation

WB(Homo sapiens, Mus musculus)

Co-IP(Homo sapiens)

WB(Homo sapiens)

Documents

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    ABclonal:Western blot - MT-ND4 Rabbit pAb (A9941)}

    Western blot - MT-ND4 Rabbit pAb (A9941)

    Western blot analysis of various lysates using MT-ND4 Rabbit pAb (A9941) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A9941? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)

    HRP-conjugated Goat anti-Rabbit IgG (H+L)
    AS014

    WBDBIHC-PELISA

    HRP-conjugated Goat anti-Rabbit IgG (H+L)

    FITC-conjugated Goat anti-Rabbit IgG (H+L)
    AS011

    IF/ICCFC

    FITC-conjugated Goat anti-Rabbit IgG (H+L)

    Cy3-conjugated Goat anti-Rabbit IgG (H+L)
    AS007

    IF/ICCFC

    Cy3-conjugated Goat anti-Rabbit IgG (H+L)

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
    AS053

    IF/ICCFC

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain
    AS061

    WBIP

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain

    HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
    AS063

    WB

    HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

    ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
    AS039

    IF/ICCFC

    ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

    Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
    AS040

    IHC-PIF/ICCFC

    Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

    ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
    AS060

    IF/ICCFC

    ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

    Goat Anti-Rabbit IgG(H+L)
    AS070

    WBELISACUT&Tag

    Goat Anti-Rabbit IgG(H+L)

    HRP-conjugated Donkey anti-Rabbit IgG (H+L)
    AS038

    WBIHC-P

    HRP-conjugated Donkey anti-Rabbit IgG (H+L)

    ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
    AS035

    IF/ICCFC

    ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
    AS073

    IF/ICCFC

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

    ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
    AS058

    IF/ICCFC

    ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

    FITC-conjugated Donkey anti-Rabbit IgG (H+L)
    AS042

    IF/ICCFC

    FITC-conjugated Donkey anti-Rabbit IgG (H+L)

    ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
    AS056

    IF/ICCFC

    ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

    AP-conjugated Goat anti-Rabbit IgG (H+L)
    AS009

    WB

    AP-conjugated Goat anti-Rabbit IgG (H+L)

    HRP-conjugated Mouse anti-Rabbit IgG (H+L)
    AS079

    WB

    HRP-conjugated Mouse anti-Rabbit IgG (H+L)

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
    AS126

    IPELISA

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

    PE Mouse anti-Rabbit IgG, light chain specific mAb
    AS129

    FC

    PE Mouse anti-Rabbit IgG, light chain specific mAb

    HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
    AS122

    WBELISA

    HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

    Rabbit Anti-Chicken IgY (H+L)
    AS090

    WB

    Rabbit Anti-Chicken IgY (H+L)

    Rabbit Anti-Chicken IgY Fab
    AS088

    WB

    Rabbit Anti-Chicken IgY Fab

    FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
    AS083

    IF/ICCFC

    FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

    Rabbit Anti-Chicken IgY (H+L)
    AS082

    WB

    Rabbit Anti-Chicken IgY (H+L)

    ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
    AS072

    IF/ICCFC

    ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)