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MYH14 Rabbit mAb (A21933)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - MYH14 Rabbit mAb (A21933)

Western blot analysis of lysates from mouse lung, using MYH14 Rabbit mAb (A21933) at1:20000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - MYH14 Rabbit mAb (A21933)

Western blot analysis of various lysates using MYH14 Rabbit mAb (A21933) at1:20000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - MYH14 Rabbit mAb (A21933)

Confocal imaging of paraffin-embedded Human colon cancer using MYH14 Rabbit mAb (A21933, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - MYH14 Rabbit mAb (A21933)

Confocal imaging of paraffin-embedded mouse small intestine using MYH14 Rabbit mAb (A21933, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product nameMYH14 Rabbit mAb
Catalog No.A21933
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC53864
This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-14 (MYO14). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene result in one form of autosomal dominant hearing impairment. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 600-700 of human MYH14 (NP_079005.3).
SequenceAGKVDYKANEWLMKNMDPLNDNVAALLHQSTDRLTAEIWKDVEGIVGLEQVSSLGDGPPGGRPRRGMFRTVGQLYKESLSRLMATLSNTNPSFVRCIVPNH
Gene ID
Swiss Prot
SynonymsDFNA4; MHC16; MYH17; PNMHH; DFNA4A; myosin; FP17425; NMHC II-C; NMHC-II-C; MYH14
Calculated MW228kDa
Observed MW250kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:20000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMCF7, Mouse lung, Rat lung
Cellular locationcytosol, extracellular exosome, growth cone
Customer validation

IF(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - MYH14 Rabbit mAb (A21933)}

Western blot - MYH14 Rabbit mAb (A21933)

Western blot analysis of lysates from mouse lung, using MYH14 Rabbit mAb (A21933) at1:20000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - MYH14 Rabbit mAb (A21933)}

Western blot - MYH14 Rabbit mAb (A21933)

Western blot analysis of various lysates using MYH14 Rabbit mAb (A21933) at1:20000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunofluorescence - MYH14 Rabbit mAb (A21933)}

Immunofluorescence - MYH14 Rabbit mAb (A21933)

Confocal imaging of paraffin-embedded Human colon cancer using MYH14 Rabbit mAb (A21933, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - MYH14 Rabbit mAb (A21933)}

Immunofluorescence - MYH14 Rabbit mAb (A21933)

Confocal imaging of paraffin-embedded mouse small intestine using MYH14 Rabbit mAb (A21933, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (26)