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MYLK Rabbit mAb (A3835)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - MYLK Rabbit mAb (A3835)

Western blot analysis of extracts of various cell lines, using MYLK Rabbit mAb (A3835) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameMYLK Rabbit mAb
Catalog No.A3835
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0842
This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1800-1900 of human MYLK (Q15746).
SequenceVAEEKPHVKPYFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVE
Gene ID
Swiss Prot
SynonymsKRP; AAT7; MLCK; MLCK1; MMIHS; MYLK1; MMIHS1; MYLK-L; smMLCK; MLCK108; MLCK210; MSTP083
Calculated MW211kDa
Observed MW130kDa/140kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesPC-3, RD, Rat lung
Cellular locationCell projection, Cleavage furrow, Cytoplasm, cytoskeleton, lamellipodium
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - MYLK Rabbit mAb (A3835)}

Western blot - MYLK Rabbit mAb (A3835)

Western blot analysis of extracts of various cell lines, using MYLK Rabbit mAb (A3835) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A3835? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)