제품 > 항체 > 다중클론항체(pAb)

MYMK Rabbit pAb (A18158)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - MYMK Rabbit pAb (A18158)

Western blot analysis of lysates from Mouse skeletal muscle, using MYMK Rabbit pAb (A18158) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameMYMK Rabbit pAb
Catalog No.A18158
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Involved in myoblast fusion. Located in plasma membrane. Implicated in Carey-Fineman-Ziter syndrome.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 120-221 of human MYMK (NP_001073952.1).
SequenceGPIGTAILIIAAKWLQKMKEKKGLYPDKSVYTQQIGPGLCFGALALMLRFFFEDWDYTYVHSFYHCALAMSFVLLLPKVNKKAGSPGTPAKLDCSTLCCACV
Gene ID
Swiss Prot
SynonymsTMEM8C; TMEM226; MYOMAKER; MYMK
Calculated MW25kDa
Observed MW27kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse skeletal muscle
Cellular locationplasma membrane.
Customer validation

WB(Mus musculus)

    ABclonal:Western blot - MYMK Rabbit pAb (A18158)}

    Western blot - MYMK Rabbit pAb (A18158)

    Western blot analysis of lysates from Mouse skeletal muscle, using MYMK Rabbit pAb (A18158) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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