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Mannose Receptor/CD206 Rabbit mAb (A21014)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from Mouse spleen, using Mannose Receptor/CD206 Rabbit mAb (A21014) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - Mannose Receptor/CD206 Rabbit mAb (A21014)

Immunohistochemistry analysis of paraffin-embedded Mouse liver using Mannose Receptor/CD206 Rabbit mAb (A21014) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat liver using Mannose Receptor/CD206 Rabbit mAb (A21014) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Mannose Receptor/CD206 Rabbit mAb (A21014)

Confocal imaging of paraffin-embedded mouse liver using Mannose Receptor/CD206 Rabbit mAb (A21014, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Confocal imaging of paraffin-embedded rat lung using Mannose Receptor/CD206 Rabbit mAb (A21014, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product name	Mannose Receptor/CD206 Rabbit mAb
Catalog No.	A21014
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC3149

Background

The recognition of complex carbohydrate structures on glycoproteins is an important part of several biological processes, including cell-cell recognition, serum glycoprotein turnover, and neutralization of pathogens. The protein encoded by this gene is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. The protein has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment.

Immunogen information

Immunogen	A synthetic peptide around C-terminal of Mouse MRC1
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	MMR; hMR; CD206; MRC1L1; CLEC13D; CLEC13DL; bA541I19.1; Mannose Receptor/CD206
Calculated MW	166kDa
Observed MW	190-250kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: 10 mM sodium HEPES and 150 mM NaCl with 0.02% Sodium azide, 100 μg/ml BSA, 50% Glycerol, pH 7.5
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	Mouse spleen
Cellular location	Cell membrane, Endosome membrane, Single-pass type I membrane protein.
Customer validation	WB(Homo sapiens) IHC(Mus musculus)