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MonoMethyl-NONO/p54nrb-R357/R365 Rabbit pAb (A8087)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Immunofluorescence - MonoMethyl-NONO/p54nrb-R357/R365 Rabbit pAb (A8087)

Immunofluorescence analysis of HeLa cells using MonoMethyl-NONO/p54nrb-R357/R365 pAb (A8087) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameMonoMethyl-NONO/p54nrb-R357/R365 Rabbit pAb
Catalog No.A8087
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16.
ImmunogenA synthetic monomethylated peptide around R357 & R365 of human NONO/p54nrb (Q15233).
SequenceRQQEEMMRR
Gene ID
Swiss Prot
SynonymsP54; NMT55; NRB54; MRXS34; P54NRB; PPP1R114
Calculated MW54kDa
Observed MWRefer to figures
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples
Cellular locationNucleus, Nucleus speckle, nucleolus

Documents

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ABclonal:Immunofluorescence - MonoMethyl-NONO/p54nrb-R357/R365 Rabbit pAb (A8087)}

Immunofluorescence - MonoMethyl-NONO/p54nrb-R357/R365 Rabbit pAb (A8087)

Immunofluorescence analysis of HeLa cells using MonoMethyl-NONO/p54nrb-R357/R365 pAb (A8087) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)