제품 > 항체 > 단일클론항체(mAb)

Mras Rabbit mAb (A20958)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Mras Rabbit mAb (A20958)

Western blot analysis of extracts of various cell lines, using Mras antibody (A20958) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameMras Rabbit mAb
Catalog No.A20958
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2946
This gene encodes a member of the Ras family of small GTPases. These membrane-associated proteins function as signal transducers in multiple processes including cell growth and differentiation, and dysregulation of Ras signaling has been associated with many types of cancer. The encoded protein may play a role in the tumor necrosis factor-alpha and MAP kinase signaling pathways. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 109-208 of human Mras (O14807).
SequenceLILRVKDRESFPMILVANKVDLMHLRKITREQGKEMATKHNIPYIETSAKDPPLNVDKAFHDLVRVIRQQIPEKSQKKKKKTKWRGDRATGTHKLQCVIL
Gene ID
Swiss Prot
SynonymsNS11; M-RAs; RRAS3; R-RAS3
Calculated MW24kDa
Observed MW24kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, C6, SH-SY5Y, Mouse lung, Mouse brain, Rat brain, Mouse kidney
Cellular locationCell membrane

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ABclonal:Western blot - Mras Rabbit mAb (A20958)}

Western blot - Mras Rabbit mAb (A20958)

Western blot analysis of extracts of various cell lines, using Mras antibody (A20958) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)