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Myogenin Rabbit pAb (A17427)

Publications (16) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Myogenin Rabbit pAb (A17427)

Western blot analysis of various lysates, using Myogenin Rabbit pAb (A17427) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - Myogenin Rabbit pAb (A17427)

Immunofluorescence analysis of NIH/3T3 cells using Myogenin Rabbit pAb (A17427) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameMyogenin Rabbit pAb
Catalog No.A17427
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100-200 of human Myogenin (NP_002470.2).
SequenceAFEALKRSTLLNPNQRLPKVEILRSAIQYIERLQALLSSLNQEERDLRYRGGGGPQPGVPSECSSHSASCSPEWGSALEFSANPGDHLLTADPTDAHNLHSLTSIVDSITVEDVSVAFPDETMPN
Gene ID
Swiss Prot
SynonymsMYF4; myf-4; bHLHc3; Myogenin
Calculated MW25kDa
Observed MW25kDa/40kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesNIH/3T3, Rat heart
Cellular locationNucleus.
Customer validation

IHC(Equus caballus)

WB(Mus musculus, Merycoidodon gracilis, Homo sapiens, Gallus gallus, Sus scrofa, Danio rerio)

IF(Mus musculus)

ChIP(Homo sapiens)

IF(Gallus gallus, Sus scrofa)

Documents

Certificate of Compliance

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ABclonal:Western blot - Myogenin Rabbit pAb (A17427)}

Western blot - Myogenin Rabbit pAb (A17427)

Western blot analysis of various lysates, using Myogenin Rabbit pAb (A17427) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - Myogenin Rabbit pAb (A17427)}

Immunofluorescence - Myogenin Rabbit pAb (A17427)

Immunofluorescence analysis of NIH/3T3 cells using Myogenin Rabbit pAb (A17427) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A17427? Please let us know so that we can cite the reference in this datasheet.