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N4BP1 Rabbit pAb (A8474)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - N4BP1 Rabbit pAb (A8474)

Western blot analysis of various lysates using N4BP1 Rabbit pAb (A8474) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - N4BP1 Rabbit pAb (A8474)

Immunofluorescence analysis of U2OS cells using N4BP1 Rabbit pAb (A8474) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameN4BP1 Rabbit pAb
Catalog No.A8474
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable mRNA binding activity; ribonuclease activity; and ubiquitin binding activity. Involved in cellular response to UV; negative regulation of macromolecule metabolic process; and regulation of innate immune response. Located in PML body; cytosol; and nucleolus. Orthologous to human N4BP1 (NEDD4 binding protein 1).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 190-590 of mouse N4BP1 (NP_085040.2).
SequenceTQGEESLFETDDDVITVGDVRPPEYTQSAATGPSSARDEVVVQEDSRNKARTPVSELTKHMDTVFSSSPDVLFVPVNGLSPDEDALSKDRVCHKRRSSDTEERHTKKQFSLENVPEGELLPDGKGSAGNEVIDLSDPASNSTNLSPDGKDTTEEMEYNILVNFFKTMGYSQEIVEKVIREYGPSTEPLLLLEEIEKENKRLQEDRDFPPCTVYPDASQSRNAGVGSTTNELTADSTPKKAQSHTEQSMVERFSQLPFKDSKHCTSNCKVNSFRTVPVGQKQEIWGSKQNSSCTVDLETDGHSASAASASPKDISFVSRGASGHQQRNPAFPENGFQQQTEPLLPNNTKPACEKRSGSCSSPQPKPNYPPLSPPLPLPQLLPSVTEARLGGSSDHIDSSVTG
Gene ID
Swiss Prot
SynonymsN4BP1
Calculated MW100kDa
Observed MW120kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesJurkat, 293T, Mouse brain, Mouse pancreas
Cellular locationNucleus, PML body, nucleolus
Customer validation

WB(Chlorocebus aethiops)

Documents

Certificate of Compliance

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    ABclonal:Western blot - N4BP1 Rabbit pAb (A8474)}

    Western blot - N4BP1 Rabbit pAb (A8474)

    Western blot analysis of various lysates using N4BP1 Rabbit pAb (A8474) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunofluorescence - N4BP1 Rabbit pAb (A8474)}

    Immunofluorescence - N4BP1 Rabbit pAb (A8474)

    Immunofluorescence analysis of U2OS cells using N4BP1 Rabbit pAb (A8474) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A8474? Please let us know so that we can cite the reference in this datasheet.