제품 > 항체 > 다중클론항체(pAb)

N4BP1 Rabbit pAb (A8474)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of various lysates using N4BP1 Rabbit pAb (A8474) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - N4BP1 Rabbit pAb (A8474)

Immunofluorescence analysis of U2OS cells using N4BP1 Rabbit pAb (A8474) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	N4BP1 Rabbit pAb
Catalog No.	A8474
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Predicted to enable mRNA binding activity; ribonuclease activity; and ubiquitin binding activity. Involved in cellular response to UV; negative regulation of macromolecule metabolic process; and regulation of innate immune response. Located in PML body; cytosol; and nucleolus. Orthologous to human N4BP1 (NEDD4 binding protein 1).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 190-590 of mouse N4BP1 (NP_085040.2).
Sequence	TQGEESLFETDDDVITVGDVRPPEYTQSAATGPSSARDEVVVQEDSRNKARTPVSELTKHMDTVFSSSPDVLFVPVNGLSPDEDALSKDRVCHKRRSSDTEERHTKKQFSLENVPEGELLPDGKGSAGNEVIDLSDPASNSTNLSPDGKDTTEEMEYNILVNFFKTMGYSQEIVEKVIREYGPSTEPLLLLEEIEKENKRLQEDRDFPPCTVYPDASQSRNAGVGSTTNELTADSTPKKAQSHTEQSMVERFSQLPFKDSKHCTSNCKVNSFRTVPVGQKQEIWGSKQNSSCTVDLETDGHSASAASASPKDISFVSRGASGHQQRNPAFPENGFQQQTEPLLPNNTKPACEKRSGSCSSPQPKPNYPPLSPPLPLPQLLPSVTEARLGGSSDHIDSSVTG
Gene ID
Swiss Prot
Synonyms	N4BP1
Calculated MW	100kDa
Observed MW	120kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200
Storage buffer	Store at 4℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	Jurkat, 293T, Mouse brain, Mouse pancreas
Cellular location	Nucleus, PML body, nucleolus
Customer validation	WB(Chlorocebus aethiops)