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N4BP1 Rabbit pAb (A8474)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - N4BP1 Rabbit pAb (A8474)

Western blot analysis of various lysates using N4BP1 Rabbit pAb (A8474) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - N4BP1 Rabbit pAb (A8474)

Immunofluorescence analysis of U2OS cells using N4BP1 Rabbit pAb (A8474) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameN4BP1 Rabbit pAb
Catalog No.A8474
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable mRNA binding activity; ribonuclease activity; and ubiquitin binding activity. Involved in cellular response to UV; negative regulation of macromolecule metabolic process; and regulation of innate immune response. Located in PML body; cytosol; and nucleolus. Orthologous to human N4BP1 (NEDD4 binding protein 1).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 190-590 of mouse N4BP1 (NP_085040.2).
SequenceTQGEESLFETDDDVITVGDVRPPEYTQSAATGPSSARDEVVVQEDSRNKARTPVSELTKHMDTVFSSSPDVLFVPVNGLSPDEDALSKDRVCHKRRSSDTEERHTKKQFSLENVPEGELLPDGKGSAGNEVIDLSDPASNSTNLSPDGKDTTEEMEYNILVNFFKTMGYSQEIVEKVIREYGPSTEPLLLLEEIEKENKRLQEDRDFPPCTVYPDASQSRNAGVGSTTNELTADSTPKKAQSHTEQSMVERFSQLPFKDSKHCTSNCKVNSFRTVPVGQKQEIWGSKQNSSCTVDLETDGHSASAASASPKDISFVSRGASGHQQRNPAFPENGFQQQTEPLLPNNTKPACEKRSGSCSSPQPKPNYPPLSPPLPLPQLLPSVTEARLGGSSDHIDSSVTG
Gene ID
Swiss Prot
SynonymsN4BP1
Calculated MW100kDa
Observed MW120kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesJurkat, 293T, Mouse brain, Mouse pancreas
Cellular locationNucleus, PML body, nucleolus
Customer validation

WB(Chlorocebus aethiops)

Documents

Certificate of Compliance

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    ABclonal:Western blot - N4BP1 Rabbit pAb (A8474)}

    Western blot - N4BP1 Rabbit pAb (A8474)

    Western blot analysis of various lysates using N4BP1 Rabbit pAb (A8474) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunofluorescence - N4BP1 Rabbit pAb (A8474)}

    Immunofluorescence - N4BP1 Rabbit pAb (A8474)

    Immunofluorescence analysis of U2OS cells using N4BP1 Rabbit pAb (A8474) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A8474? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)