Western blot - NCAM Rabbit PolymAb® (A27193PM)
Western blot analysis of various lysates using NCAM Rabbit PolymAb® (A27193PM) at 1:16000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot - NCAM Rabbit PolymAb® (A27193PM)
Western blot analysis of various lysates using NCAM Rabbit PolymAb® (A27193PM) at 1:16000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): MCF7
Exposure time: 30s.
Immunohistochemistry - NCAM Rabbit PolymAb® (A27193PM)
Immunohistochemistry analysis of paraffin-embedded Human brain tissue using NCAM Rabbit PolymAb® (A27193PM) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry - NCAM Rabbit PolymAb® (A27193PM)
Immunohistochemistry analysis of paraffin-embedded Human appendix tissue using NCAM Rabbit PolymAb® (A27193PM) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry - NCAM Rabbit PolymAb® (A27193PM)
Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using NCAM Rabbit PolymAb® (A27193PM) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry - NCAM Rabbit PolymAb® (A27193PM)
Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using NCAM Rabbit PolymAb® (A27193PM) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry - NCAM Rabbit PolymAb® (A27193PM)
Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using NCAM Rabbit PolymAb® (A27193PM) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunofluorescence - NCAM Rabbit PolymAb® (A27193PM)
Confocal imaging of SH-SY5Y cells using NCAM Rabbit PolymAb® (A27193PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Immunofluorescence - NCAM Rabbit PolymAb® (A27193PM)
Confocal imaging of paraffin-embedded Mouse brain tissue using NCAM Rabbit PolymAb® (A27193PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Immunofluorescence - NCAM Rabbit PolymAb® (A27193PM)
Confocal imaging of paraffin-embedded Rat brain tissue using NCAM Rabbit PolymAb® (A27193PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.