제품 > 항체 > 다중클론항체(pAb)

NCOR1 Rabbit pAb (A7046)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - NCOR1 Rabbit pAb (A7046)

Western blot analysis of lysates from Jurkat cells, using NCOR1 Rabbit pAb (A7046) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - NCOR1 Rabbit pAb (A7046)

Immunohistochemistry analysis of paraffin-embedded Rat stomach using NCOR1 Rabbit pAb (A7046) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - NCOR1 Rabbit pAb (A7046)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using NCOR1 Rabbit pAb (A7046) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - NCOR1 Rabbit pAb (A7046)

Immunohistochemistry analysis of paraffin-embedded Mouse testis using NCOR1 Rabbit pAb (A7046) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - NCOR1 Rabbit pAb (A7046)

Immunofluorescence analysis of U2OS cells using NCOR1 Rabbit pAb (A7046). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameNCOR1 Rabbit pAb
Catalog No.A7046
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that mediates ligand-independent transcription repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. It is part of a complex which also includes histone deacetylases and transcriptional regulators similar to the yeast protein Sin3p. This gene is located between the Charcot-Marie-Tooth and Smith-Magenis syndrome critical regions on chromosome 17. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 17 and 20.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human NCOR1 (NP_006302.2).
SequenceMSSSGYPPNQGAFSTEQSRYPPHSVQYTFPNTRHQQEFAVPDYRSSHLEVSQASQLLQQQQQQQLRRRPSLLSEFHPGSDRPQERRTSYEPFHPGPSPVDHDSLESKRPRLEQVSDSHFQRVSAAVLPLVHPLPEGLRASADAKKDPAFGGKHEAPSSPISGQPCGDDQNASPSKLSKEELIQSMDRVDREIAKVEQQIL
Gene ID
Swiss Prot
SynonymsN-CoR; TRAC1; N-CoR1; hN-CoR; PPP1R109; NCOR1
Calculated MW270kDa
Observed MW270kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:100
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesJurkat
Cellular locationNucleus.
Customer validation

IF(Mus musculus)

WB(Drosophila melanogaster,Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - NCOR1 Rabbit pAb (A7046)}

Western blot - NCOR1 Rabbit pAb (A7046)

Western blot analysis of lysates from Jurkat cells, using NCOR1 Rabbit pAb (A7046) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - NCOR1 Rabbit pAb (A7046)}

Immunohistochemistry - NCOR1 Rabbit pAb (A7046)

Immunohistochemistry analysis of paraffin-embedded Rat stomach using NCOR1 Rabbit pAb (A7046) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - NCOR1 Rabbit pAb (A7046)}

Immunohistochemistry - NCOR1 Rabbit pAb (A7046)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using NCOR1 Rabbit pAb (A7046) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - NCOR1 Rabbit pAb (A7046)}

Immunohistochemistry - NCOR1 Rabbit pAb (A7046)

Immunohistochemistry analysis of paraffin-embedded Mouse testis using NCOR1 Rabbit pAb (A7046) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - NCOR1 Rabbit pAb (A7046)}

Immunofluorescence - NCOR1 Rabbit pAb (A7046)

Immunofluorescence analysis of U2OS cells using NCOR1 Rabbit pAb (A7046). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A7046? Please let us know so that we can cite the reference in this datasheet.