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NLRP1 Rabbit pAb (A16212)

Publications (6) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - NLRP1 Rabbit pAb (A16212)

Western blot analysis of lysates from K-562 cells, using NLRP1 Rabbit pAb (A16212) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - NLRP1 Rabbit pAb (A16212)

Immunohistochemistry analysis of paraffin-embedded human colon using NLRP1 Rabbit pAb (A16212) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NLRP1 Rabbit pAb (A16212)

Immunohistochemistry analysis of paraffin-embedded human tonsil using NLRP1 Rabbit pAb (A16212) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - NLRP1 Rabbit pAb (A16212)

Immunofluorescence analysis of K-562 cells using NLRP1 Rabbit pAb (A16212) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - NLRP1 Rabbit pAb (A16212)

Immunofluorescence analysis of PC-12 cells using NLRP1 Rabbit pAb (A16212) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - NLRP1 Rabbit pAb (A16212)

Immunofluorescence analysis of SH-SY5Y cells using NLRP1 Rabbit pAb (A16212) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameNLRP1 Rabbit pAb
Catalog No.A16212
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the Ced-4 family of apoptosis proteins. Ced-family members contain a caspase recruitment domain (CARD) and are known to be key mediators of programmed cell death. The encoded protein contains a distinct N-terminal pyrin-like motif, which is possibly involved in protein-protein interactions. This protein interacts strongly with caspase 2 and weakly with caspase 9. Overexpression of this gene was demonstrated to induce apoptosis in cells. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity of some variants has not been determined.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1370-1473 of human NLRP1 (NP_127497.1).
SequencePSPLDAPQLLHFVDQYREQLIARVTSVEVVLDKLHGQVLSQEQYERVLAENTRPSQMRKLFSLSQSWDRKCKDGLYQALKETHPHLIMELWEKGSKKGLLPLSS
Gene ID
Swiss Prot
SynonymsNAC; JRRP; MSPC; AIADK; CARD7; CIDED; NALP1; SLEV1; DEFCAP; PP1044; VAMAS1; CLR17.1; DEFCAP-L/S; NLRP1
Calculated MW166kDa
Observed MW160kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesK-562
Cellular locationcytoplasm, cytosol, inflammasome complex, NLRP1 inflammasome complex, nucleolus, nucleoplasm, nucleus
Customer validation

WB(Mus musculus, Sus scrofa, Rattus norvegicus)

IF(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - NLRP1 Rabbit pAb (A16212)}

Western blot - NLRP1 Rabbit pAb (A16212)

Western blot analysis of lysates from K-562 cells, using NLRP1 Rabbit pAb (A16212) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - NLRP1 Rabbit pAb (A16212)}

Immunohistochemistry - NLRP1 Rabbit pAb (A16212)

Immunohistochemistry analysis of paraffin-embedded human colon using NLRP1 Rabbit pAb (A16212) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - NLRP1 Rabbit pAb (A16212)}

Immunohistochemistry - NLRP1 Rabbit pAb (A16212)

Immunohistochemistry analysis of paraffin-embedded human tonsil using NLRP1 Rabbit pAb (A16212) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - NLRP1 Rabbit pAb (A16212)}

Immunofluorescence - NLRP1 Rabbit pAb (A16212)

Immunofluorescence analysis of K-562 cells using NLRP1 Rabbit pAb (A16212) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NLRP1 Rabbit pAb (A16212)}

Immunofluorescence - NLRP1 Rabbit pAb (A16212)

Immunofluorescence analysis of PC-12 cells using NLRP1 Rabbit pAb (A16212) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NLRP1 Rabbit pAb (A16212)}

Immunofluorescence - NLRP1 Rabbit pAb (A16212)

Immunofluorescence analysis of SH-SY5Y cells using NLRP1 Rabbit pAb (A16212) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A16212? Please let us know so that we can cite the reference in this datasheet.

Secondary Antibodies (26)