제품 > 항체 > 다중클론항체(pAb)

NLRX1 Rabbit pAb (A4976)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from Mouse heart, using NLRX1 Rabbit pAb (A4976) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunoprecipitation - NLRX1 Rabbit pAb (A4976)

Immunoprecipitation analysis of 600 μg extracts of Mouse kidney using 3 μg NLRX1 antibody (A4976). Western blot was performed from the immunoprecipitate using NLRX1 (A4976) at a dilution of 1:1000.

Review

Overview

Product name	NLRX1 Rabbit pAb
Catalog No.	A4976
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a member of the NLR family and localizes to the outer mitochondrial membrane. The encoded protein is a regulator of mitochondrial antivirus responses. Three transcript variants encoding the same protein have been found for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 87-360 of human NLRX1 (NP_078894.2).
Sequence	AEWFSRLPREERQFGPTFALDTVHVDPVIRESTPDELLRPPAELALEHQPPQAGLPPLALSQLFNPDACGRRVQTVVLYGTVGTGKSTLVRKMVLDWCYGRLPAFELLIPFSCEDLSSLGPAPASLCQLVAQRYTPLKEVLPLMAAAGSHLLFVLHGLEHLNLDFRLAGTGLCSDPEEPQEPAAIIVNLLRKYMLPQASILVTTRPSAIGRIPSKYVGRYGEICGFSDTNLQKLYFQLRLNQPYCGYAVGGSGVSATPAQRDHLVQMLSRNLEG
Gene ID
Swiss Prot
Synonyms	NOD5; NOD9; NOD26; DLNB26; CLR11.3; NLRX1
Calculated MW	108kDa
Observed MW	108kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IP 0.5μg-4μg antibody for 400μg-600μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunoprecipitation
Positive samples	Mouse heart
Cellular location	Mitochondrion outer membrane.
Customer validation	(293T、HepG2) WB(Mus musculus)

Documents

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Research Area

NLRX1 Rabbit pAb images

Western blot - NLRX1 Rabbit pAb (A4976)

Immunoprecipitation - NLRX1 Rabbit pAb (A4976)

Immunoprecipitation analysis of 600 μg extracts of Mouse kidney using 3 μg NLRX1 antibody (A4976). Western blot was performed from the immunoprecipitate using NLRX1 (A4976) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.