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N-Myc/MYCN Rabbit pAb (A0499)

Review (1)Publications (6) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - N-Myc/MYCN Rabbit pAb (A0499)

Western blot analysis of lysates from HeLa cells, using N-Myc/MYCN Rabbit pAb (A0499) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - N-Myc/MYCN Rabbit pAb (A0499)

Immunohistochemistry analysis of paraffin-embedded mouse brain using N-Myc/MYCN Rabbit pAb (A0499) (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - N-Myc/MYCN Rabbit pAb (A0499)

Immunohistochemistry analysis of paraffin-embedded rat brain using N-Myc/MYCN Rabbit pAb (A0499) (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - N-Myc/MYCN Rabbit pAb (A0499)

Immunofluorescence analysis of paraffin-embedded mouse brain using N-Myc/MYCN Rabbit pAb (A0499) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - N-Myc/MYCN Rabbit pAb (A0499)

Immunofluorescence analysis of paraffin-embedded rat brain using N-Myc/MYCN Rabbit pAb (A0499) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for N-Myc/MYCN Rabbit pAb(A0499)

Overview

Product nameN-Myc/MYCN Rabbit pAb
Catalog No.A0499
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 168-267 of human N-Myc/MYCN (NP_005369.2).
SequenceAGRAGAALPAELAHPAAECVDPAVVFPFPVNKREPAPVPAAPASAPAAGPAVASGAGIAAPAGAPGVAPPRPGGRQTSGGDHKALSTSGEDTLSDSDDED
Gene ID
Swiss Prot
SynonymsNMYC; ODED; MODED; N-myc; bHLHe37; MYCNsORF; MYCNsPEP; N-Myc/MYCN
Calculated MW50kDa
Observed MW62kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa
Cellular locationNucleus
Customer validation

(Human neuroblastoma)

WB(Homo sapiens, Rattus norvegicus)

IF(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - N-Myc/MYCN Rabbit pAb (A0499)}

Western blot - N-Myc/MYCN Rabbit pAb (A0499)

Western blot analysis of lysates from HeLa cells, using N-Myc/MYCN Rabbit pAb (A0499) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - N-Myc/MYCN Rabbit pAb (A0499)}

Immunohistochemistry - N-Myc/MYCN Rabbit pAb (A0499)

Immunohistochemistry analysis of paraffin-embedded mouse brain using N-Myc/MYCN Rabbit pAb (A0499) (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - N-Myc/MYCN Rabbit pAb (A0499)}

Immunohistochemistry - N-Myc/MYCN Rabbit pAb (A0499)

Immunohistochemistry analysis of paraffin-embedded rat brain using N-Myc/MYCN Rabbit pAb (A0499) (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - N-Myc/MYCN Rabbit pAb (A0499)}

Immunofluorescence - N-Myc/MYCN Rabbit pAb (A0499)

Immunofluorescence analysis of paraffin-embedded mouse brain using N-Myc/MYCN Rabbit pAb (A0499) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - N-Myc/MYCN Rabbit pAb (A0499)}

Immunofluorescence - N-Myc/MYCN Rabbit pAb (A0499)

Immunofluorescence analysis of paraffin-embedded rat brain using N-Myc/MYCN Rabbit pAb (A0499) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

Secondary Antibodies (26)