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NOD1 Rabbit pAb (A1246)

Publications (6) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - NOD1 Rabbit pAb (A1246)

Western blot analysis of various lysates using NOD1 Rabbit pAb (A1246) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 120s.

ABclonal:Immunohistochemistry - NOD1 Rabbit pAb (A1246)

Immunohistochemistry analysis of paraffin-embedded Human mammary cancer using NOD1 Rabbit pAb (A1246) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - NOD1 Rabbit pAb (A1246)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using NOD1 Rabbit pAb (A1246) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - NOD1 Rabbit pAb (A1246)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using NOD1 Rabbit pAb (A1246) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product nameNOD1 Rabbit pAb
Catalog No.A1246
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) family of proteins. The encoded protein plays a role in innate immunity by acting as a pattern-recognition receptor (PRR) that binds bacterial peptidoglycans and initiates inflammation. This protein has also been implicated in the immune response to viral and parasitic infection. Major structural features of this protein include an N-terminal caspase recruitment domain (CARD), a centrally located nucleotide-binding domain (NBD), and 10 tandem leucine-rich repeats (LRRs) in its C terminus. The CARD is involved in apoptotic signaling, LRRs participate in protein-protein interactions, and mutations in the NBD may affect the process of oligomerization and subsequent function of the LRR domain. Mutations in this gene are associated with asthma, inflammatory bowel disease, Behcet disease and sarcoidosis in human patients.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human NOD1 (NP_006083.1).
SequenceMEEQGHSEMEIIPSESHPHIQLLKSNRELLVTHIRNTQCLVDNLLKNDYFSAEDAEIVCACPTQPDKVRKILDLVQSKGEEVSEFFLYLLQQLADAYVDLRPWLLEIGFSPSLLTQSKVVVNTDPVSRYTQQLRHHLGRDSKFVLCYAQKEELLLEEIYMDTIMELVGFSNESLGSLNSLACLLDHTTGILNEQGETIFILGDAGVGKSMLLQRLQSLWATGRLDAGVKFFFHFRCRMFSCFKESDRLCLQDLLFKHYCYPERDPEEVFA
Gene ID
Swiss Prot
SynonymsCARD4; NLRC1; CLR7.1; NOD1
Calculated MW108kDa
Observed MW108kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesLO2, 293T
Cellular locationApical cell membrane, Basolateral cell membrane, Cell membrane, Cytoplasm.
Customer validation

WB(Mus musculus, Homo sapiens)

IHC(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - NOD1 Rabbit pAb (A1246)}

Western blot - NOD1 Rabbit pAb (A1246)

Western blot analysis of various lysates using NOD1 Rabbit pAb (A1246) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 120s.
ABclonal:Immunohistochemistry - NOD1 Rabbit pAb (A1246)}

Immunohistochemistry - NOD1 Rabbit pAb (A1246)

Immunohistochemistry analysis of paraffin-embedded Human mammary cancer using NOD1 Rabbit pAb (A1246) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - NOD1 Rabbit pAb (A1246)}

Immunohistochemistry - NOD1 Rabbit pAb (A1246)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using NOD1 Rabbit pAb (A1246) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - NOD1 Rabbit pAb (A1246)}

Immunohistochemistry - NOD1 Rabbit pAb (A1246)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using NOD1 Rabbit pAb (A1246) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A1246? Please let us know so that we can cite the reference in this datasheet.

Secondary Antibodies (26)