제품 > 항체 > 다중클론항체(pAb)

NRIP1 Rabbit pAb (A9955)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - NRIP1 Rabbit pAb (A9955)

Western blot analysis of extracts of Mouse brain, using NRIP1 Polyclonal Antibody (A9955) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

Overview

Product nameNRIP1 Rabbit pAb
Catalog No.A9955
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Nuclear receptor interacting protein 1 (NRIP1) is a nuclear protein that specifically interacts with the hormone-dependent activation domain AF2 of nuclear receptors. Also known as RIP140, this protein modulates transcriptional activity of the estrogen receptor.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 939-1158 of human NRIP1 (NP_003480.2).
SequenceLLLSENCVRDLSPHRSNSVADSKKKGHKNNVTNSKPEFSISSLNGLMYSSTQPSSCMDNRTFSYPGVVKTPVSPTFPEHLGCAGSRPESGLLNGCSMPSEKGPIKWVITDAEKNEYEKDSPRLTKTNPILYYMLQKGGNSVTSRETQDKDIWREASSAESVSQVTAKEELLPTAETKASFFNLRSPYNSHMGNNASRPHSANGEVYGLLGSVLTIKKESE
Gene ID
Swiss Prot
SynonymsCAKUT3; RIP140
Calculated MW127kDa
Observed MW150kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse brain
Cellular locationNucleus
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - NRIP1 Rabbit pAb (A9955)}

Western blot - NRIP1 Rabbit pAb (A9955)

Western blot analysis of extracts of Mouse brain, using NRIP1 Polyclonal Antibody (A9955) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A9955? Please let us know so that we can cite the reference in this datasheet.

Secondary Antibodies (26)