제품 > 항체 > 다중클론항체(pAb)

NSFL1C Rabbit pAb (A6677)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using NSFL1C Rabbit pAb (A6677) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - NSFL1C Rabbit pAb (A6677)

Immunofluorescence analysis of HeLa cells using NSFL1C Rabbit pAb (A6677). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - NSFL1C Rabbit pAb (A6677)

Immunoprecipitation analysis of 200 μg extracts of A-549 cells, using 3 μg NSFL1C antibody (A6677). Western blot was performed from the immunoprecipitate using NSFL1C antibody (A6677) at a dilution of 1:1000.

Overview

Product name	NSFL1C Rabbit pAb
Catalog No.	A6677
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human NSFL1C (NP_057227.2).
Sequence	MAAERQEALREFVAVTGAEEDRARFFLESAGWDLQIALASFYEDGGDEDIVTISQATPSSVSRGTAPSDNRVTSFRDLIHDQDEDEEEEEGQRFYAGGSERSGQQIVGPPRKKSPNELVDDLFKGAKEHGAVAVERVTKSPGETSKPRPFAGGGYRLGAAPEEESAYVAGEKRQHSSQDVHVVLK
Gene ID
Swiss Prot
Synonyms	P47; UBX1; UBXD10; UBXN2C; dJ776F14.1; NSFL1C
Calculated MW	41kDa
Observed MW	41kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Immunoprecipitation
Positive samples	293T, U-251MG, Mouse kidney, Mouse brain, Rat brain, Rat kidney
Cellular location	Chromosome, Golgi apparatus, Golgi stack, Nucleus
Customer validation	WB(Homo sapiens)

Documents

Certificate of Compliance

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Research Area

NSFL1C Rabbit pAb images

Western blot - NSFL1C Rabbit pAb (A6677)

Immunofluorescence - NSFL1C Rabbit pAb (A6677)

Immunofluorescence analysis of HeLa cells using NSFL1C Rabbit pAb (A6677). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunoprecipitation - NSFL1C Rabbit pAb (A6677)

* For research use only. Not for therapeutic or diagnostic purposes.