제품 > 항체 > 다중클론항체(pAb)

NTMT1 Rabbit pAb (A7098)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of extracts of various cell lines, using NTMT1 antibody (A7098) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - NTMT1 Rabbit pAb (A7098)

Immunofluorescence analysis of A-549 cells using NTMT1 antibody (A7098). Blue: DAPI for nuclear staining.

Overview

Product name	NTMT1 Rabbit pAb
Catalog No.	A7098
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The METTL11A gene encodes an N-terminal methyltransferase for the RAN (MIM 601179) guanine nucleotide exchange factor regulator of chromosome condensation 1 (RCC1; MIM 179710). METTL11A enzyme alpha-N-methylates other protein targets such as SET (MIM 600960) and RB (MIM 180200).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-223 of human NTMT1 (NP_054783.2).
Sequence	MTSEVIEDEKQFYSKAKTYWKQIPPTVDGMLGGYGHISSIDINSSRKFLQRFLREGPNKTGTSCALDCGAGIGRITKRLLLPLFREVDMVDITEDFLVQAKTYLGEEGKRVRNYFCCGLQDFTPEPDSYDVIWIQWVIGHLTDQHLAEFLRRCKGSLRPNGIIVIKDNMAQEGVILDDVDSSVCRDLDVVRRIICSAGLSLLAEERQENLPDEIYHVYSFALR
Gene ID
Swiss Prot
Synonyms	NRMT; NRMT1; NTM1A; AD-003; HOMT1A; C9orf32; METTL11A
Calculated MW	25kDa
Observed MW	25kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:100
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	HeLa, SKOV3, U-87MG, A-431, Raji, Mouse brain, Rat ovary
Cellular location	Nucleus
Customer validation	WB(Homo sapiens) IF(Mus musculus) WB(Mus musculus)