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NTMT1 Rabbit pAb (A7098)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - NTMT1 Rabbit pAb (A7098)

Western blot analysis of extracts of various cell lines, using NTMT1 antibody (A7098) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - NTMT1 Rabbit pAb (A7098)

Immunofluorescence analysis of A-549 cells using NTMT1 antibody (A7098). Blue: DAPI for nuclear staining.

Overview

Product nameNTMT1 Rabbit pAb
Catalog No.A7098
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The METTL11A gene encodes an N-terminal methyltransferase for the RAN (MIM 601179) guanine nucleotide exchange factor regulator of chromosome condensation 1 (RCC1; MIM 179710). METTL11A enzyme alpha-N-methylates other protein targets such as SET (MIM 600960) and RB (MIM 180200).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-223 of human NTMT1 (NP_054783.2).
SequenceMTSEVIEDEKQFYSKAKTYWKQIPPTVDGMLGGYGHISSIDINSSRKFLQRFLREGPNKTGTSCALDCGAGIGRITKRLLLPLFREVDMVDITEDFLVQAKTYLGEEGKRVRNYFCCGLQDFTPEPDSYDVIWIQWVIGHLTDQHLAEFLRRCKGSLRPNGIIVIKDNMAQEGVILDDVDSSVCRDLDVVRRIICSAGLSLLAEERQENLPDEIYHVYSFALR
Gene ID
Swiss Prot
SynonymsNRMT; NRMT1; NTM1A; AD-003; HOMT1A; C9orf32; METTL11A
Calculated MW25kDa
Observed MW25kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa, SKOV3, U-87MG, A-431, Raji, Mouse brain, Rat ovary
Cellular locationNucleus
Customer validation

WB(Homo sapiens)

IF(Mus musculus)

WB(Mus musculus)

Documents

Certificate of Compliance

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    ABclonal:Western blot - NTMT1 Rabbit pAb (A7098)}

    Western blot - NTMT1 Rabbit pAb (A7098)

    Western blot analysis of extracts of various cell lines, using NTMT1 antibody (A7098) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Immunofluorescence - NTMT1 Rabbit pAb (A7098)}

    Immunofluorescence - NTMT1 Rabbit pAb (A7098)

    Immunofluorescence analysis of A-549 cells using NTMT1 antibody (A7098). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A7098? Please let us know so that we can cite the reference in this datasheet.