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NUDEL/NDEL1 Rabbit pAb (A5776)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - NUDEL/NDEL1 Rabbit pAb (A5776)

Western blot analysis of extracts of various cell lines, using NUDEL/NUDEL/NDEL1 antibody (A5776) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameNUDEL/NDEL1 Rabbit pAb
Catalog No.A5776
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables identical protein binding activity. Involved in chromosome segregation; positive regulation of GTPase activity; and regulation of intracellular protein transport. Located in kinetochore. Biomarker of schizophrenia.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-345 of human NUDEL/NUDEL/NDEL1 (NP_110435.1).
SequenceMDGEDIPDFSSLKEETAYWKELSLKYKQSFQEARDELVEFQEGSRELEAELEAQLVQAEQRNRDLQADNQRLKYEVEALKEKLEHQYAQSYKQVSVLEDDLSQTRAIKEQLHKYVRELEQANDDLERAKRATIVSLEDFEQRLNQAIERNAFLESELDEKESLLVSVQRLKDEARDLRQELAVRERQQEVTRKSAPSSPTLDCEKMDSAVQASLSLPATPVGKGTENTFPSPKAIPNGFGTSPLTPSARISALNIVGDLLRKVGALESKLAACRNFAKDQASRKSYISGNVNCGVLNGNGTKFSRSGHTSFFDKGAVNGFDPAPPPPGLGSSRPSSAPGMLPLSV
Gene ID
Swiss Prot
SynonymsEOPA; NDE2; NUDEL; MITAP1; NDE1L1
Calculated MW38kDa
Observed MW39kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesU-87MG, Mouse brain
Cellular locationChromosome, Cytoplasm, centromere, centrosome, cytoskeleton, kinetochore, microtubule organizing center, spindle
Customer validation

WB(Mus musculus)

Documents

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ABclonal:Western blot - NUDEL/NDEL1 Rabbit pAb (A5776)}

Western blot - NUDEL/NDEL1 Rabbit pAb (A5776)

Western blot analysis of extracts of various cell lines, using NUDEL/NUDEL/NDEL1 antibody (A5776) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

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