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OLA1 Rabbit pAb (A11671)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - OLA1 Rabbit pAb (A11671)

Western blot analysis of various lysates using OLA1 Rabbit pAb (A11671) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - OLA1 Rabbit pAb (A11671)

Immunohistochemistry analysis of paraffin-embedded human breast cancer using OLA1 Rabbit pAb (A11671) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - OLA1 Rabbit pAb (A11671)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using OLA1 Rabbit pAb (A11671) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - OLA1 Rabbit pAb (A11671)

Immunofluorescence analysis of NIH/3T3 cells using OLA1 Rabbit pAb (A11671) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - OLA1 Rabbit pAb (A11671)

Immunofluorescence analysis of PC-12 cells using OLA1 Rabbit pAb (A11671) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameOLA1 Rabbit pAb
Catalog No.A11671
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the GTPase protein family. The encoded protein interacts with breast cancer-associated gene 1 (BRCA1) and BRCA1-associated RING domain protein (BARD1), and is involved in centrosome regulation. Overexpression of this gene has been observed in multiple types of cancer and may be associated with poor survival. Pseudogenes of this gene have been defined on chromosomes 17 and 22.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 130-310 of human OLA1 (NP_037473.3).
SequenceDDITHVEGSVDPIRDIEIIHEELQLKDEEMIGPIIDKLEKVAVRGGDKKLKPEYDIMCKVKSWVIDQKKPVRFYHDWNDKEIEVLNKHLFLTSKPMVYLVNLSEKDYIRKKNKWLIKIKEWVDKYDPGALVIPFSGALELKLQELSAEERQKYLEANMTQSALPKIIKAGFAALQLEYFFT
Gene ID
Swiss Prot
SynonymsDOC45; GBP45; GTBP9; GTPBP9; PTD004; OLA1
Calculated MW45kDa
Observed MW45kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesU-251MG, Jurkat, MCF7, Mouse brain, Mouse lung, Rat testis
Cellular locationCytoplasm, Nucleus, nucleolus
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - OLA1 Rabbit pAb (A11671)}

    Western blot - OLA1 Rabbit pAb (A11671)

    Western blot analysis of various lysates using OLA1 Rabbit pAb (A11671) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunohistochemistry - OLA1 Rabbit pAb (A11671)}

    Immunohistochemistry - OLA1 Rabbit pAb (A11671)

    Immunohistochemistry analysis of paraffin-embedded human breast cancer using OLA1 Rabbit pAb (A11671) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - OLA1 Rabbit pAb (A11671)}

    Immunohistochemistry - OLA1 Rabbit pAb (A11671)

    Immunohistochemistry analysis of paraffin-embedded human lung cancer using OLA1 Rabbit pAb (A11671) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunofluorescence - OLA1 Rabbit pAb (A11671)}

    Immunofluorescence - OLA1 Rabbit pAb (A11671)

    Immunofluorescence analysis of NIH/3T3 cells using OLA1 Rabbit pAb (A11671) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - OLA1 Rabbit pAb (A11671)}

    Immunofluorescence - OLA1 Rabbit pAb (A11671)

    Immunofluorescence analysis of PC-12 cells using OLA1 Rabbit pAb (A11671) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A11671? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)