제품 > 항체 > 다중클론항체(pAb)

ORAI1 Rabbit pAb (A7412)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ORAI1 Rabbit pAb (A7412)

Western blot analysis of various lysates using (A7412) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - ORAI1 Rabbit pAb (A7412)

Western blot analysis of various lysates using (A7412) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product nameORAI1 Rabbit pAb
Catalog No.A7412
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a membrane calcium channel subunit that is activated by the calcium sensor STIM1 when calcium stores are depleted. This type of channel is the primary way for calcium influx into T-cells. Defects in this gene are a cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 1 (IDTICED1).
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human ORAI1 (NP_116179.2).
SequenceMHPEPAPPPSRSSPELPPSGGSTTSGSRRSRRRSGDGEPPGAPPPPPSAVTYPDWIGQSYSEVMSLNEHSMQALSWRKLYLSRAKLKASSRTSALLSGFA
Gene ID
Swiss Prot
SynonymsIMD9; TAM2; ORAT1; CRACM1; TMEM142A; ORAI1
Calculated MW33kDa
Observed MW32-55kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, MCF7, Mouse lung, Mouse testis, Mouse spleen, Rat lung, Rat liver
Cellular locationCell membrane, Cytoplasmic vesicle, Multi-pass membrane protein, autophagosome
Customer validation

WB(Rattus norvegicus, Gallus gallus)

Documents

Certificate of Compliance

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ABclonal:Western blot - ORAI1 Rabbit pAb (A7412)}

Western blot - ORAI1 Rabbit pAb (A7412)

Western blot analysis of various lysates using (A7412) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - ORAI1 Rabbit pAb (A7412)}

Western blot - ORAI1 Rabbit pAb (A7412)

Western blot analysis of various lysates using (A7412) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)