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OSR1 Rabbit pAb (A18272)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - OSR1 Rabbit pAb (A18272)

Western blot analysis of various lysates using OSR1 Rabbit pAb (A18272) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - OSR1 Rabbit pAb (A18272)

Immunohistochemistry analysis of paraffin-embedded rat kidney using OSR1 Rabbit pAb (A18272) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - OSR1 Rabbit pAb (A18272)

Immunohistochemistry analysis of paraffin-embedded human esophageal using OSR1 Rabbit pAb (A18272) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - OSR1 Rabbit pAb (A18272)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using OSR1 Rabbit pAb (A18272) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameOSR1 Rabbit pAb
Catalog No.A18272
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables sequence-specific double-stranded DNA binding activity. Involved in negative regulation of ion transmembrane transporter activity; positive regulation of gastrulation; and pronephros development. Located in nucleus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 28-115 of human OSR1 (NP_660303.1).
SequenceNGLPTVPSDHLPNLYGFSALHAVHLHQWTLGYPAMHLPRSSFSKVPGTVSSLVDARFQLPAFPWFPHVIQPKPEITAGGSVPALKTKP
Gene ID
Swiss Prot
SynonymsODD; OSR1
Calculated MW30kDa
Observed MW30kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesNCI-H460, Cal-27, A-549, 22Rv1
Cellular locationcytosol, nucleus
Customer validation

WB(Homo sapiens)

IHC(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - OSR1 Rabbit pAb (A18272)}

Western blot - OSR1 Rabbit pAb (A18272)

Western blot analysis of various lysates using OSR1 Rabbit pAb (A18272) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - OSR1 Rabbit pAb (A18272)}

Immunohistochemistry - OSR1 Rabbit pAb (A18272)

Immunohistochemistry analysis of paraffin-embedded rat kidney using OSR1 Rabbit pAb (A18272) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - OSR1 Rabbit pAb (A18272)}

Immunohistochemistry - OSR1 Rabbit pAb (A18272)

Immunohistochemistry analysis of paraffin-embedded human esophageal using OSR1 Rabbit pAb (A18272) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - OSR1 Rabbit pAb (A18272)}

Immunohistochemistry - OSR1 Rabbit pAb (A18272)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using OSR1 Rabbit pAb (A18272) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A18272? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)