Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat
Western blot analysis of extracts of various cell lines, using Olig2 Rabbit mAb (A4555) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
| Product name | Olig2 Rabbit mAb |
|---|---|
| Catalog No. | A4555 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC1032 |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Olig2 (Q13516). |
|---|---|
| Sequence | AATAHPAAAAHAAHHPAVHHPILPPAAAAAAAAAAAAAVSSASLPGSGLPSVGSIRPPHGLLKSPSAAAAAPLGGGGGGSGASGGFQHWGGMPCPCSMCQV |
| Gene ID | |
| Swiss Prot | |
| Synonyms | BHLHB1; OLIGO2; RACK17; PRKCBP2; bHLHe19 |
| Calculated MW | 32kDa |
| Observed MW | 40kDa |
| Reactivity | Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | C6, Mouse brain |
| Cellular location | Cytoplasm, Nucleus |
| Customer validation | WB(Mus musculus) IHC(Rattus norvegicus) IF(Rattus norvegicus) |
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* For research use only. Not for therapeutic or diagnostic purposes.
Publishing research using A4555? Please let us know so that we can cite the reference in this datasheet.
