제품 > 항체 > 다중클론항체(pAb)

P2RY14 Rabbit pAb (A8178)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - P2RY14 Rabbit pAb (A8178)

Western blot analysis of lysates from K-562 cells, using P2RY14 Rabbit pAb (A8178) at 1:1500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - P2RY14 Rabbit pAb (A8178)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using P2RY14 Rabbit pAb (A8178) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameP2RY14 Rabbit pAb
Catalog No.A8178
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The product of this gene belongs to the family of G-protein coupled receptors, which contains several receptor subtypes with different pharmacological selectivity for various adenosine and uridine nucleotides. This receptor is a P2Y purinergic receptor for UDP-glucose and other UDP-sugars coupled to G-proteins. It has been implicated in extending the known immune system functions of P2Y receptors by participating in the regulation of the stem cell compartment, and it may also play a role in neuroimmune function. Two transcript variants encoding the same protein have been identified for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 209-338 of human P2RY14 (NP_055694.3).
SequenceITKKIFKSHLKSSRNSTSVKKKSSRNIFSIVFVFFVCFVPYHIARIPYTKSQTEAHYSCQSKEILRYMKEFTLLLSAANVCLDPIIYFFLCQPFREILCKKLHIPLKAQNDLDISRIKRGNTTLESTDTL
Gene ID
Swiss Prot
SynonymsP2Y14; BPR105; GPR105; P2RY14
Calculated MW39kDa
Observed MW38kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesK-562
Cellular locationCell membrane, Multi-pass membrane protein
Customer validation

WB(Mus musculus)

IF(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - P2RY14 Rabbit pAb (A8178)}

Western blot - P2RY14 Rabbit pAb (A8178)

Western blot analysis of lysates from K-562 cells, using P2RY14 Rabbit pAb (A8178) at 1:1500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - P2RY14 Rabbit pAb (A8178)}

Immunohistochemistry - P2RY14 Rabbit pAb (A8178)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using P2RY14 Rabbit pAb (A8178) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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