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PADI1/PAD1 Rabbit mAb (A23951)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - PADI1/PAD1 Rabbit mAb (A23951)

Western blot analysis of lysates from HaCaT cells using PADI1/PAD1 Rabbit mAb (A23951) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - PADI1/PAD1 Rabbit mAb (A23951)

Western blot analysis of lysates from Mouse ovary using PADI1/PAD1 Rabbit mAb (A23951) at 1:1400 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
Exposure time: 60 s.

ABclonal:Western blot - PADI1/PAD1 Rabbit mAb (A23951)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with PADI1/PAD1 using PADI1/PAD1 Rabbit mAb (A23951) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 20 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
Exposure time: 1 s.

Overview

Product namePADI1/PAD1 Rabbit mAb
Catalog No.A23951
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC61569
This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. The type I enzyme is involved in the late stages of epidermal differentiation, where it deiminates filaggrin and keratin K1, which maintains hydration of the stratum corneum, and hence the cutaneous barrier function. This enzyme may also play a role in hair follicle formation. This gene exists in a cluster with four other paralogous genes.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-277 of human PADI1/PAD1(NP_037490.2).
SequenceMAPKRVVQLSLKMPTHAVCVVGVEAHVDIHSDVPKGANSFRVSGSSGVEVFMVYNRTRVKEPIGKARWPLDTDADMVVSVGTASKELKDFKVRVSYFGEQEDQALGRSVLYLTGVDISLEVDTGRTGKVKRSQGDKKTWRWGPEGYGAILLVNCDRDNHRSAEPDLTHSWLMSLADLQDMSPMLLSCNGPDKLFDSHKLVLNVPFSDSKRVRVFCARGGNSLSDYKQVLGPQCLSYEVERQPGEQEIKFYVEGLTFPDADFLGLVSLSVSLVDPGTL
Gene ID
Swiss Prot
SynonymsPADI1; HPAD10; PAD1; PDI; PDI1; peptidyl arginine deiminase 1; PADI1/PAD1
Calculated MW74kDa
Observed MW75kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHaCaT, 293T transfected with PADI1/PAD1, Mouse ovary
Cellular locationCytoplasm.

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ABclonal:Western blot - PADI1/PAD1 Rabbit mAb (A23951)}

Western blot - PADI1/PAD1 Rabbit mAb (A23951)

Western blot analysis of lysates from HaCaT cells using PADI1/PAD1 Rabbit mAb (A23951) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - PADI1/PAD1 Rabbit mAb (A23951)}

Western blot - PADI1/PAD1 Rabbit mAb (A23951)

Western blot analysis of lysates from Mouse ovary using PADI1/PAD1 Rabbit mAb (A23951) at 1:1400 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
Exposure time: 60 s.
ABclonal:Western blot - PADI1/PAD1 Rabbit mAb (A23951)}

Western blot - PADI1/PAD1 Rabbit mAb (A23951)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with PADI1/PAD1 using PADI1/PAD1 Rabbit mAb (A23951) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 20 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
Exposure time: 1 s.

* For research use only. Not for therapeutic or diagnostic purposes.

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