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PAH Rabbit mAb (A20949)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PAH Rabbit mAb (A20949)

Western blot analysis of various lysates using PAH Rabbit mAb (A20949) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - PAH Rabbit mAb (A20949)

Western blot analysis of various lysates using PAH Rabbit mAb (A20949) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - PAH Rabbit mAb (A20949)

Immunofluorescence analysis of HepG2 cells using PAH Rabbit mAb (A20949) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PAH Rabbit mAb (A20949)

Immunofluorescence analysis of paraffin-embedded mouse liver using PAH Rabbit mAb (A20949) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PAH Rabbit mAb (A20949)

Immunofluorescence analysis of paraffin-embedded rat liver using PAH Rabbit mAb (A20949) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namePAH Rabbit mAb
Catalog No.A20949
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2936
This gene encodes a member of the biopterin-dependent aromatic amino acid hydroxylase protein family. The encoded phenylalanine hydroxylase enzyme hydroxylates phenylalanine to tyrosine and is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human PAH (P00439).
SequenceMSTAVLENPGLGRKLSDFGQETSYIEDNCNQNGAISLIFSLKEEVGALAKVLRLFEENDVNLTHIESRPSRLKKDEYEFFTHLDKRSLPALTNIIKILRH
Gene ID
Swiss Prot
SynonymsPH; PKU; PKU1; PAH
Calculated MW52kDa
Observed MW52kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHepG2, Mouse liver, Mouse kidney, Rat liver
Cellular locationcytosol.

Documents

Certificate of Compliance

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ABclonal:Western blot - PAH Rabbit mAb (A20949)}

Western blot - PAH Rabbit mAb (A20949)

Western blot analysis of various lysates using PAH Rabbit mAb (A20949) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - PAH Rabbit mAb (A20949)}

Western blot - PAH Rabbit mAb (A20949)

Western blot analysis of various lysates using PAH Rabbit mAb (A20949) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunofluorescence - PAH Rabbit mAb (A20949)}

Immunofluorescence - PAH Rabbit mAb (A20949)

Immunofluorescence analysis of HepG2 cells using PAH Rabbit mAb (A20949) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PAH Rabbit mAb (A20949)}

Immunofluorescence - PAH Rabbit mAb (A20949)

Immunofluorescence analysis of paraffin-embedded mouse liver using PAH Rabbit mAb (A20949) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PAH Rabbit mAb (A20949)}

Immunofluorescence - PAH Rabbit mAb (A20949)

Immunofluorescence analysis of paraffin-embedded rat liver using PAH Rabbit mAb (A20949) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)