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PAX6 Rabbit mAb (A24118)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PAX6 Rabbit mAb (A24118)

Western blot analysis of various lysates using PAX6 Rabbtit mAb (A24118) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): THP-1.
Exposure time: 90s.

ABclonal:Immunohistochemistry - PAX6 Rabbit mAb (A24118)

Immunohistochemistry analysis of paraffin-embedded Rat pancreas tissue using PAX6 Rabbtit mAb (A24118) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PAX6 Rabbit mAb (A24118)

Immunohistochemistry analysis of paraffin-embedded Mouse pancreas tissue using PAX6 Rabbtit mAb (A24118) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PAX6 Rabbit mAb (A24118)

Immunohistochemistry analysis of paraffin-embedded Human pancreatic cancer tissue using PAX6 Rabbtit mAb (A24118) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PAX6 Rabbit mAb (A24118)

Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using PAX6 Rabbtit mAb (A24118) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - PAX6 Rabbit mAb (A24118)

Confocal imaging of K-562 cells using PAX6 Rabbtit mAb (A24118, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product namePAX6 Rabbit mAb
Catalog No.A24118
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC63795
This gene encodes paired box protein Pax-6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to a conserved paired box domain, a hallmark feature of this gene family, the encoded protein also contains a homeobox domain. Both domains are known to bind DNA and function as regulators of gene transcription. Activity of this protein is key in the development of neural tissues, particularly the eye. This gene is regulated by multiple enhancers located up to hundreds of kilobases distant from this locus. Mutations in this gene or in the enhancer regions can cause ocular disorders such as aniridia and Peter's anomaly. Use of alternate promoters and alternative splicing results in multiple transcript variants encoding different isoforms. Interestingly, inclusion of a particular alternate coding exon has been shown to increase the length of the paired box domain and alter its DNA binding specificity. Consequently, isoforms that carry the shorter paired box domain regulate a different set of genes compared to the isoforms carrying the longer paired box domain.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 323-422 of human PAX6 (NP_000271.1).
SequenceTNTYSALPPMPSFTMANNLPMQPPVPSQTSSYSCMLPTSPSVNGRSYDTYTPPHMQTHMNSQPMGTSGTTSTGLISPGVSVPVQVPGSEPDMSQYWPRLQ
Gene ID
Swiss Prot
SynonymsAN; PAX6
Calculated MW47kDa/48kDa
Observed MW50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC-P 1:200 - 1:800
  • IF/ICC 1:200 - 1:400
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa
Cellular locationNucleus.

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ABclonal:Western blot - PAX6 Rabbit mAb (A24118)}

Western blot - PAX6 Rabbit mAb (A24118)

Western blot analysis of various lysates using PAX6 Rabbtit mAb (A24118) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): THP-1.
Exposure time: 90s.
ABclonal:Immunohistochemistry - PAX6 Rabbit mAb (A24118)}

Immunohistochemistry - PAX6 Rabbit mAb (A24118)

Immunohistochemistry analysis of paraffin-embedded Rat pancreas tissue using PAX6 Rabbtit mAb (A24118) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PAX6 Rabbit mAb (A24118)}

Immunohistochemistry - PAX6 Rabbit mAb (A24118)

Immunohistochemistry analysis of paraffin-embedded Mouse pancreas tissue using PAX6 Rabbtit mAb (A24118) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PAX6 Rabbit mAb (A24118)}

Immunohistochemistry - PAX6 Rabbit mAb (A24118)

Immunohistochemistry analysis of paraffin-embedded Human pancreatic cancer tissue using PAX6 Rabbtit mAb (A24118) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PAX6 Rabbit mAb (A24118)}

Immunohistochemistry - PAX6 Rabbit mAb (A24118)

Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using PAX6 Rabbtit mAb (A24118) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunofluorescence - PAX6 Rabbit mAb (A24118)}

Immunofluorescence - PAX6 Rabbit mAb (A24118)

Confocal imaging of K-562 cells using PAX6 Rabbtit mAb (A24118, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 100x.

* For research use only. Not for therapeutic or diagnostic purposes.

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