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PBR/TSPO Rabbit pAb (A15649)

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Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Western blot - PBR/TSPO Rabbit pAb (A15649)

Western blot analysis of various lysates, using PBR/TSPO Rabbit pAb (A15649) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - PBR/TSPO Rabbit pAb (A15649)

Immunofluorescence analysis of L929 cells using PBR/TSPO Rabbit pAb (A15649) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namePBR/TSPO Rabbit pAb
Catalog No.A15649
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Present mainly in the mitochondrial compartment of peripheral tissues, the protein encoded by this gene interacts with some benzodiazepines and has different affinities than its endogenous counterpart. The protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis. Alternatively spliced transcript variants have been reported; one of the variants lacks an internal exon and is considered non-coding, and the other variants encode the same protein.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-50 of human PBR/PBR/TSPO (NP_000705.2).
SequenceMAPPWVPAMGFTLAPSLGCFVGSRFVHGEGLRWYAGLQKPSWHPPHWVLG
Gene ID
Swiss Prot
SynonymsDBI; IBP; MBR; PBR; PBS; BPBS; BZRP; PKBS; PTBR; mDRC; pk18; TSPO1; PBR/TSPO
Calculated MW19kDa
Observed MW19kDa
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:100
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse kidney
Cellular locationMitochondrion membrane, Multi-pass membrane protein.

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ABclonal:Western blot - PBR/TSPO Rabbit pAb (A15649)}

Western blot - PBR/TSPO Rabbit pAb (A15649)

Western blot analysis of various lysates, using PBR/TSPO Rabbit pAb (A15649) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunofluorescence - PBR/TSPO Rabbit pAb (A15649)}

Immunofluorescence - PBR/TSPO Rabbit pAb (A15649)

Immunofluorescence analysis of L929 cells using PBR/TSPO Rabbit pAb (A15649) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (1)

Secondary Antibodies (26)