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PENK Rabbit pAb (A6302)

Publications (5) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PENK Rabbit pAb (A6302)

Western blot analysis of lysates from THP-1 cells, using PENK Rabbit pAb (A6302) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 120s.

ABclonal:Immunohistochemistry - PENK Rabbit pAb (A6302)

Immunohistochemistry analysis of paraffin-embedded Human prostate using PENK Rabbit pAb (A6302) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - PENK Rabbit pAb (A6302)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using PENK Rabbit pAb (A6302) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - PENK Rabbit pAb (A6302)

Immunofluorescence analysis of paraffin-embedded Human adrenal gland using PENK Rabbit pAb (A6302) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namePENK Rabbit pAb
Catalog No.A6302
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the pentapeptide opioids Met-enkephalin and Leu-enkephalin, which are stored in synaptic vesicles, then released into the synapse where they bind to mu- and delta-opioid receptors to modulate the perception of pain. Other non-opioid cleavage products may function in distinct biological activities.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 25-267 of human PENK (NP_001129162.1).
SequenceECSQDCATCSYRLVRPADINFLACVMECEGKLPSLKIWETCKELLQLSKPELPQDGTSTLRENSKPEESHLLAKRYGGFMKRYGGFMKKMDELYPMEPEEEANGSEILAKRYGGFMKKDAEEDDSLANSSDLLKELLETGDNRERSHHQDGSDNEEEVSKRYGGFMRGLKRSPQLEDEAKELQKRYGGFMRRVGRPEWWMDYQKRYGGFLKRFAEALPSDEEGESYSKEVPEMEKRYGGFMRF
Gene ID
Swiss Prot
SynonymsPE; PENK-A; PENK
Calculated MW31kDa
Observed MW36kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesTHP-1
Cellular locationSecreted.
Customer validation

WB(Homo sapiens, Rattus norvegicus, Mus musculus)

IHC(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - PENK Rabbit pAb (A6302)}

    Western blot - PENK Rabbit pAb (A6302)

    Western blot analysis of lysates from THP-1 cells, using PENK Rabbit pAb (A6302) at 1:500 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 120s.
    ABclonal:Immunohistochemistry - PENK Rabbit pAb (A6302)}

    Immunohistochemistry - PENK Rabbit pAb (A6302)

    Immunohistochemistry analysis of paraffin-embedded Human prostate using PENK Rabbit pAb (A6302) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - PENK Rabbit pAb (A6302)}

    Immunohistochemistry - PENK Rabbit pAb (A6302)

    Immunohistochemistry analysis of paraffin-embedded Mouse brain using PENK Rabbit pAb (A6302) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunofluorescence - PENK Rabbit pAb (A6302)}

    Immunofluorescence - PENK Rabbit pAb (A6302)

    Immunofluorescence analysis of paraffin-embedded Human adrenal gland using PENK Rabbit pAb (A6302) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A6302? Please let us know so that we can cite the reference in this datasheet.

    항체 (2)

    Secondary Antibodies (26)