제품 > 항체 > 단일클론항체(mAb)

PE Rabbit anti-Mouse CD274/PD-L1 mAb (A26505)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Flow cytometry: 1X10^6 C2C12 cells (Low Expression, left) and A20 cells (right) were surface-stained with PE Rabbit anti-Mouse CD274/PD-L1 mAb (A26505, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 A20 cells were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-Mouse CD274/PD-L1 mAb (A26505, 5 μl/Test, right).

Flow cytometry: 1X10^6 C57BL/6 mouse splenocytes were surface-stained with PE Rabbit anti-Mouse CD274/PD-L1 mAb (A26505, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 C57BL/6 mouse splenocytes were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-Mouse CD274/PD-L1 mAb (A26505, 5 μl/Test, right).

Overview

Product name	PE Rabbit anti-Mouse CD274/PD-L1 mAb
Catalog No.	A26505
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC61951-PE

Background

The protein encoded by this gene is an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Mice deficient for this gene display a variety of phenotypes including decreased allogeneic fetal survival rates and severe experimental autoimmune encephalomyelitis.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 19-235 of mouse CD274/PD-L1(NP_068693.1).
Sequence	FTITAPKDLYVVEYGSNVTMECRFPVERELDLLALVVYWEKEDEQVIQFVAGEEDLKPQHSNFRGRASLPKDQLLKGNAALQITDVKLQDAGVYCCIISYGGADYKRITLKVNAPYRKINQRISVDPATSEHELICQAEGYPEAEVIWTNSDHQPVSGKRSVTTSRTEGMLLNVTSSLRVNATANDVFYCTFWRSQPGQNHTAELIIPELPATHPPQ
Gene ID
Swiss Prot
Synonyms	B7h1; Pdl1; Pdcd1l1; Pdcd1lg1; A530045L16Rik
Calculated MW	32kDa
Observed MW	Refer to figures

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell membrane.

Documents

Certificate of Compliance

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Research Area

PE Rabbit anti-Mouse CD274/PD-L1 mAb images

Flow CytoMetry - PE Rabbit anti-Mouse CD274/PD-L1 mAb (A26505)

Flow cytometry: 1X10^6 A20 cells were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-Mouse CD274/PD-L1 mAb (A26505, 5 μl/Test, right).

Flow CytoMetry - PE Rabbit anti-Mouse CD274/PD-L1 mAb (A26505)

* For research use only. Not for therapeutic or diagnostic purposes.