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PGAM5 Rabbit mAb (A22203)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PGAM5 Rabbit mAb (A22203)

Western blot analysis of various lysates, using PGAM5 Rabbit mAb (A22203) at1:20000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - PGAM5 Rabbit mAb (A22203)

Western blot analysis of lysates from Rat kidney, using PGAM5 Rabbit mAb (A22203) at1:20000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - PGAM5 Rabbit mAb (A22203)

Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using PGAM5 Rabbit mAb (A22203) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PGAM5 Rabbit mAb (A22203)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using PGAM5 Rabbit mAb (A22203) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PGAM5 Rabbit mAb (A22203)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using PGAM5 Rabbit mAb (A22203) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PGAM5 Rabbit mAb (A22203)

Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using PGAM5 Rabbit mAb (A22203) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PGAM5 Rabbit mAb (A22203)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using PGAM5 Rabbit mAb (A22203) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PGAM5 Rabbit mAb (A22203)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using PGAM5 Rabbit mAb (A22203) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - PGAM5 Rabbit mAb (A22203)

Confocal imaging of Hep G2 cells using PGAM5 Rabbit mAb (A22203, dilution 1:300)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

Overview

Product namePGAM5 Rabbit mAb
Catalog No.A22203
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC53895
Enables GTPase activator activity and protein serine/threonine phosphatase activity. Involved in necroptotic process. Located in mitochondrion.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 50-146 of human PGAM5 (NP_001164014.1).
SequenceGARPGPGVWDPNWDRREPLSLINVRKRNVESGEEELASKLDHYKAKATRHIFLIRHSQYHVDGSLEKDRTLTPLGREQAELTGLRLASLGLKFNKIV
Gene ID
Swiss Prot
SynonymsBXLBV68; PGAM5
Calculated MW32kDa/28KDa
Observed MW28kDa/32kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:20000 - 1:80000
  • IHC-P 1:100 - 1:500
  • IF/ICC 1:300 - 1:1200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHep G2, A-549, Rat kidney
Cellular locationmitochondrial outer membrane, mitochondrion.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - PGAM5 Rabbit mAb (A22203)}

    Western blot - PGAM5 Rabbit mAb (A22203)

    Western blot analysis of various lysates, using PGAM5 Rabbit mAb (A22203) at1:20000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Western blot - PGAM5 Rabbit mAb (A22203)}

    Western blot - PGAM5 Rabbit mAb (A22203)

    Western blot analysis of lysates from Rat kidney, using PGAM5 Rabbit mAb (A22203) at1:20000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - PGAM5 Rabbit mAb (A22203)}

    Immunohistochemistry - PGAM5 Rabbit mAb (A22203)

    Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using PGAM5 Rabbit mAb (A22203) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - PGAM5 Rabbit mAb (A22203)}

    Immunohistochemistry - PGAM5 Rabbit mAb (A22203)

    Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using PGAM5 Rabbit mAb (A22203) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - PGAM5 Rabbit mAb (A22203)}

    Immunohistochemistry - PGAM5 Rabbit mAb (A22203)

    Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using PGAM5 Rabbit mAb (A22203) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - PGAM5 Rabbit mAb (A22203)}

    Immunohistochemistry - PGAM5 Rabbit mAb (A22203)

    Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using PGAM5 Rabbit mAb (A22203) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - PGAM5 Rabbit mAb (A22203)}

    Immunohistochemistry - PGAM5 Rabbit mAb (A22203)

    Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using PGAM5 Rabbit mAb (A22203) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - PGAM5 Rabbit mAb (A22203)}

    Immunohistochemistry - PGAM5 Rabbit mAb (A22203)

    Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using PGAM5 Rabbit mAb (A22203) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunofluorescence - PGAM5 Rabbit mAb (A22203)}

    Immunofluorescence - PGAM5 Rabbit mAb (A22203)

    Confocal imaging of Hep G2 cells using PGAM5 Rabbit mAb (A22203, dilution 1:300)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

    * For research use only. Not for therapeutic or diagnostic purposes.

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