제품 > 항체 > 다중클론항체(pAb)

PID1 Rabbit pAb (A13220)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using PID1 Rabbit pAb (A13220) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

ABclonal:Immunoprecipitation - PID1 Rabbit pAb (A13220)

Immunoprecipitation analysis of 25 μg extracts of Mouse liver cells using 3 μg PID1 antibody (A13220). Western blot was performed from the immunoprecipitate using PID1 antibody (A13220) at a dilution of 1:1000.

Review

Overview

Product name	PID1 Rabbit pAb
Catalog No.	A13220
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Involved in several processes, including mitochondrion morphogenesis; negative regulation of phosphate metabolic process; and positive regulation of macromolecule metabolic process. Located in cytoplasm.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 124-248 of human PID1 (NP_060403.3).
Sequence	REDVFPANALLEIRPFQVWLHHLDHKGEATVHMDTFQVARIAYCTADHNVSPNIFAWVYREINDDLSYQMDCHAVECESKLEAKKLAHAMMEAFRKTFHSMKSDGRIHSNSSSEEVSQELESDDG
Gene ID
Swiss Prot
Synonyms	PCLI1; NYGGF4; P-CLI1; HMFN2073; PID1
Calculated MW	28kDa
Observed MW	27kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IP 0.5μg-4μg antibody for 25μg-100μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunoprecipitation
Positive samples	U-87MG, Mouse brain, Mouse kidney, Mouse liver, Rat brain
Cellular location	Cytoplasm
Customer validation	(ApplicationIP)