제품 > 항체 > 다중클론항체(pAb)

PIP4K2A Rabbit pAb (A7446)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using PIP4K2A Rabbit pAb (A7446) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.

ABclonal:Immunohistochemistry - PIP4K2A Rabbit pAb (A7446)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using PIP4K2A Rabbit pAb (A7446) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded Mouse lung using PIP4K2A Rabbit pAb (A7446) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - PIP4K2A Rabbit pAb (A7446)

Immunofluorescence analysis of A549 cells using PIP4K2A Rabbit pAb (A7446).Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.

Overview

Product name	PIP4K2A Rabbit pAb
Catalog No.	A7446
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Phosphatidylinositol-5, 4-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. The protein encoded by this gene is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5, 4-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 120-350 of human PIP4K2A (NP_005019.2).
Sequence	APLPNDSQARSGARFHTSYDKRYIIKTITSEDVAEMHNILKKYHQYIVECHGITLLPQFLGMYRLNVDGVEIYVIVTRNVFSHRLSVYRKYDLKGSTVAREASDKEKAKELPTLKDNDFINEGQKIYIDDNNKKVFLEKLKKDVEFLAQLKLMDYSLLVGIHDVERAEQEEVECEENDGEEEGESDGTHPVGTPPDSPGNTLNSSPPLAPGEFDPNIDVYGIKCHENSPRK
Gene ID
Swiss Prot
Synonyms	PIPK; PI5P4KA; PIP5K2A; PIP5KIIA; PIP5KII-alpha; PIP4K2A
Calculated MW	46kDa
Observed MW	46kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	Jurkat, MCF7, Mouse brain, Mouse spinal cord, Rat brain
Cellular location	Cell membrane, Cytoplasm, Nucleus
Customer validation	WB(Mus musculus)

Documents

Certificate of Compliance

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Research Area

연구세포 생물학 & 발달 생물학세포 골격액틴

PIP4K2A Rabbit pAb images

Western blot - PIP4K2A Rabbit pAb (A7446)

Immunohistochemistry - PIP4K2A Rabbit pAb (A7446)

Immunofluorescence - PIP4K2A Rabbit pAb (A7446)

Immunofluorescence analysis of A549 cells using PIP4K2A Rabbit pAb (A7446).Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.

* For research use only. Not for therapeutic or diagnostic purposes.