제품 > 항체 > 다중클론항체(pAb)

PIWIL2 Rabbit pAb (A6044)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - PIWIL2 Rabbit pAb (A6044)

Western blot analysis of extracts of various cell lines, using PIWIL2 antibody (A6044) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Overview

Product namePIWIL2 Rabbit pAb
Catalog No.A6044
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables RNA binding activity; endoribonuclease activity; and polysome binding activity. Involved in several processes, including germ-line stem cell population maintenance; nucleic acid metabolic process; and positive regulation of cellular protein metabolic process. Acts upstream of or within oogenesis and positive regulation of meiosis I. Located in several cellular components, including cytoplasmic ribonucleoprotein granule; dense body; and perinucleolar chromocenter. Part of PET complex. Is expressed in central nervous system; genitourinary system; and sensory organ. Orthologous to human PIWIL2 (piwi like RNA-mediated gene silencing 2).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 398-652 of mouse PIWIL2 (Q8CDG1).
SequenceQNKEHFQDECSKLLVGSIVITRYNNRTYRIDDVDWNKTPKDSFVMSDGKEITFLEYYSKNYGITVKEDDQPLLIHRPSERQNNHGMLLKGEILLLPELSFMTGIPEKMKKDFRAMKDLTQQINLSPKQHHGALECLLQRISQNETASNELTRWGLSLHKDVHKIEGRLLPMERINLRNTSFVTSEDLNWVKEVTRDASILTIPMHFWALFYPKRAMDQARELVNMLEKIAGPIGMRISPPAWVELKDDRIETYIR
Gene ID
Swiss Prot
Synonymsmili; Piwil1l
Calculated MW105kDa/109kDa
Observed MW110kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:3000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse skeletal muscle, Rat skeletal muscle
Cellular locationCytoplasm
Customer validation

WB(Rattus norvegicus)

    ABclonal:Western blot - PIWIL2 Rabbit pAb (A6044)}

    Western blot - PIWIL2 Rabbit pAb (A6044)

    Western blot analysis of extracts of various cell lines, using PIWIL2 antibody (A6044) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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