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PKC alpha + beta + gamma Rabbit mAb (A24003)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PKC alpha + beta + gamma Rabbit mAb (A24003)

Western blot analysis of various lysates, using PKC alpha + beta + gamma Rabbit mAb (A24003) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

Overview

Product namePKC alpha + beta + gamma Rabbit mAb
Catalog No.A24003
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC3211
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 300-500 of human PKC alpha + beta + gamma (NP_002728.2/NP_002729.2/NP_002730.1).
SequenceELRQKFERAKISQGTKVPEEKTTNTVSKFDNNGNRDRMKLTDFNFLMVLGKGSFGKVMLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFYAAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKT
Gene ID
Swiss Prot
SynonymsAAG6; PKCA; PRKACA; PKCI+/-; PKCalpha; PKC-alpha; PKC alpha + beta + gamma
Calculated MW78kDa
Observed MW78kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:8000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, Mouse lung, Rat lung
Cellular locationCell membrane, Cytoplasm, Mitochondrion membrane, Nucleus, Peripheral membrane protein.

Documents

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    ABclonal:Western blot - PKC alpha + beta + gamma Rabbit mAb (A24003)}

    Western blot - PKC alpha + beta + gamma Rabbit mAb (A24003)

    Western blot analysis of various lysates, using PKC alpha + beta + gamma Rabbit mAb (A24003) at 1:2000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 20s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)