제품 > 항체 > 다중클론항체(pAb)

PLVAP Rabbit pAb (A15906)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - PLVAP Rabbit pAb (A15906)

Western blot analysis of various lysates using PLVAP Rabbit pAb (A15906) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product namePLVAP Rabbit pAb
Catalog No.A15906
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable identical protein binding activity. Involved in MAPK cascade; positive regulation of cellular extravasation; and tumor necrosis factor-mediated signaling pathway. Located in cell surface. Colocalizes with caveola. Implicated in congenital diarrhea.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 63-442 of human PLVAP (NP_112600.1).
SequenceTERRAEGLYSQLLGLTASQSNLTKELNFTTRAKDAIMQMWLNARRDLDRINASFRQCQGDRVIYTNNQRYMAAIILSEKQCRDQFKDMNKSCDALLFMLNQKVKTLEVEIAKEKTICTKDKESVLLNKRVAEEQLVECVKTRELQHQERQLAKEQLQKVQALCLPLDKDKFEMDLRNLWRDSIIPRSLDNLGYNLYHPLGSELASIRRACDHMPSLMSSKVEELARSLRADIERVARENSDLQRQKLEAQQGLRASQEAKQKVEKEAQAREAKLQAECSRQTQLALEEKAVLRKERDNLAKELEEKKREAEQLRMELAIRNSALDTCIKTKSQPMMPVSRPMGPVPNPQPIDPASLEEFKRKILESQRPPAGIPVAPSSG
Gene ID
Swiss Prot
SynonymsPV1; FELS; PV-1; gp68; DIAR10; PLVAP
Calculated MW51kDa
Observed MW55kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse lung, Rat lung, Rat kidney
Cellular locationCell membrane, Cytoplasm, Membrane, Single-pass type II membrane protein, caveola, perinuclear region
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - PLVAP Rabbit pAb (A15906)}

Western blot - PLVAP Rabbit pAb (A15906)

Western blot analysis of various lysates using PLVAP Rabbit pAb (A15906) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A15906? Please let us know so that we can cite the reference in this datasheet.

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