제품 > 항체 > 단일클론항체(mAb)

PNMT Rabbit mAb (A20862)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of extracts of various cell lines, using PNMT antibody (A20862) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

ABclonal:Immunofluorescence - PNMT Rabbit mAb (A20862)

Immunofluorescence analysis of Human adrenal gland cells using PNMT Rabbit mAb (A20862) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product name	PNMT Rabbit mAb
Catalog No.	A20862
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC2786

Background

The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. Alternatively spliced transcript variants have been found for this gene.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 183-282 of human PNMT (P11086).
Sequence	CLEAVSPDLASFQRALDHITTLLRPGGHLLLIGALEESWYLAGEARLTVVPVSEEEVREALVRSGYKVRDLRTYIMPAHLQTGVDDVKGVFFAWAQKVGL
Gene ID
Swiss Prot
Synonyms	PENT; PNMTase
Calculated MW	31kDa
Observed MW	31kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	K-562, HepG2, Mouse kidney, Rat kidney
Cellular location	Cytosol
Customer validation	WB(Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

PNMT Rabbit mAb images

Western blot - PNMT Rabbit mAb (A20862)

Immunofluorescence - PNMT Rabbit mAb (A20862)

Immunofluorescence analysis of Human adrenal gland cells using PNMT Rabbit mAb (A20862) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.