제품 > 항체 > 다중클론항체(pAb)

POP5 Rabbit pAb (A16530)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - POP5 Rabbit pAb (A16530)

Western blot analysis of various lysates using POP5 Rabbit pAb (A16530) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product namePOP5 Rabbit pAb
Catalog No.A16530
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables ribonuclease P RNA binding activity. Contributes to ribonuclease P activity. Involved in tRNA 5'-leader removal. Located in nucleolus. Part of multimeric ribonuclease P complex and ribonuclease MRP complex.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 50-150 of human POP5 (NP_057002.2).
SequenceSIGFAVRYLNAYTGIVLLRCRKEFYQLVWSALPFITYLENKGHRYPCFFNTLHVGGTIRTCQKFLIQYNRRQLLILLQNCTDEGEREAIQKSVTRSCLLEE
Gene ID
Swiss Prot
SynonymsRPP2; RPP20; hPop5; HSPC004; POP5
Calculated MW19kDa
Observed MW19kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesA-549, K562, 293T
Cellular locationNucleus, nucleolus

Documents

Certificate of Compliance

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    ABclonal:Western blot - POP5 Rabbit pAb (A16530)}

    Western blot - POP5 Rabbit pAb (A16530)

    Western blot analysis of various lysates using POP5 Rabbit pAb (A16530) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.