제품 > 항체 > 다중클론항체(pAb)

PRICKLE2 Rabbit pAb (A8023)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PRICKLE2 Rabbit pAb (A8023)

Western blot analysis of extracts of various cell lines, using PRICKLE2 antibody (A8023) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - PRICKLE2 Rabbit pAb (A8023)

Immunofluorescence analysis of A549 cells using PRICKLE2 antibody (A8023).

Overview

Product namePRICKLE2 Rabbit pAb
Catalog No.A8023
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a homolog of Drosophila prickle. The exact function of this gene is not known, however, studies in mice suggest that it may be involved in seizure prevention. Mutations in this gene are associated with progressive myoclonic epilepsy type 5.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 615-844 of human PRICKLE2 (NP_942559.1).
SequenceQEMEGNLHQLSNPIGYRDLQSHGRMHQSFDFDGGMAGSKLPGQEGVRIQPMSERTRRRATSRDDNRRFRPHRSRRSRRSRSDNALHLASEREAISRLKDRPPLRAREDYDQFMRQRSFQESMGHGSRRDLYGQCPRTVSDLALQNAFGDRWGPYFAEYDWCSTCSSSSESDNEGYFLGEPIPQPARLRYVTSDELLHKYSSYGLPKSSTLGGRGQLHSRKRQKSKNCIIS
Gene ID
Swiss Prot
SynonymsEPM5
Calculated MW96kDa
Observed MW126kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse brain, Rat brain
Cellular locationNucleus membrane
Customer validation

Other(Oryctolagus cuniculus)

Documents

Certificate of Compliance

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    ABclonal:Western blot - PRICKLE2 Rabbit pAb (A8023)}

    Western blot - PRICKLE2 Rabbit pAb (A8023)

    Western blot analysis of extracts of various cell lines, using PRICKLE2 antibody (A8023) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunofluorescence - PRICKLE2 Rabbit pAb (A8023)}

    Immunofluorescence - PRICKLE2 Rabbit pAb (A8023)

    Immunofluorescence analysis of A549 cells using PRICKLE2 antibody (A8023).

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A8023? Please let us know so that we can cite the reference in this datasheet.